Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/1/29 |
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| Summary: | Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from the bacterium <i>Cobetia marina</i> KMM 1449, isolated from a marine sediment sample collected along the shore of the Sea of Japan. The CPS was isolated by saline solution, purified by a series of chromatographic procedures, and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The following new structure of the CPS from <i>C. marina</i> KMM 1449 was established and consisted of sulfated and simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rha<i>p</i>2S-(1→3)-β-D-Man<i>p</i>6PGro-(1→. To elucidate the genetic basis of the CPS biosynthesis, the whole genomic sequence of <i>C. marina</i> KMM 1449 was obtained. The CPS biosynthetic gene cluster (BGC) of about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha and GDP-Man), assembly (GTs genes), translocation (ABC transporter genes), sulfation (PAPS biosynthesis and sulfotransferase genes) and lipid carrier biosynthesis (<i>wcb</i> operon). Comparative analysis of the CPS BGCs from available <i>Cobetia</i> genomes showed the presence of KMM 1449-like CPS BGC among strains of all three <i>Cobetia</i> species. The study of new natural sulfated polysaccharides, as well as the elucidation of the pathways of their biosynthesis, provides the basis for the development of potential anticancer drugs. |
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| ISSN: | 1660-3397 |