Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments
Abstract Magnetoliposomes containing magnetite, soy lecithin, stigmasterol, and beta-sitosterol of the mean size minor than 160 nm were obtained by a scalable and green process using autoclave and sonication without organic solvents. The formation, size of the liposome, linkage, and encapsulation of...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-82480-x |
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| author | Rosangela Maria Ferreira da Costa e Silva Ângela Leão Andrade Erico Tadeu Fraga Freitas Thalita Marcolan Valverde Luciano Roni Silva Lara Darliane Aparecida Martins Jorge Luis Lopez Humberto Osório Stumpf Clésia Cristina Nascentes Alfredo Miranda de Goes Rosana Zacarias Domingues |
| author_facet | Rosangela Maria Ferreira da Costa e Silva Ângela Leão Andrade Erico Tadeu Fraga Freitas Thalita Marcolan Valverde Luciano Roni Silva Lara Darliane Aparecida Martins Jorge Luis Lopez Humberto Osório Stumpf Clésia Cristina Nascentes Alfredo Miranda de Goes Rosana Zacarias Domingues |
| author_sort | Rosangela Maria Ferreira da Costa e Silva |
| collection | DOAJ |
| description | Abstract Magnetoliposomes containing magnetite, soy lecithin, stigmasterol, and beta-sitosterol of the mean size minor than 160 nm were obtained by a scalable and green process using autoclave and sonication without organic solvents. The formation, size of the liposome, linkage, and encapsulation of the magnetite were evaluated by Cryo-TEM. The stability of magnetoliposomes after storage for 6 months at 4 °C was improved by liposome size, the ability of soy lecithin to preserve the magnetite phase against oxidation, pH, polydispersity index, and zeta potential. The iron oxide phase stability was assessed using no conventional X-ray diffraction (high-resolution transmission electron microscopy), energy loss electron spectroscopy, and selected area electron diffraction) in time zero (fresh sample) and 6 months. The high zeta potential measured for magnetoliposomes, │53│ mV, indicated a low tendency to agglomerate. Lip-Fe3O4@lecithin with concentrations of 0.58 mg mL−1 of liposome showed high cell viability and are potential candidates for drug delivery and hyperthermia treatments in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. |
| format | Article |
| id | doaj-art-62054b065b7c410ca5fa42c7f75f015e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-62054b065b7c410ca5fa42c7f75f015e2025-01-26T12:26:31ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-82480-xFormation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatmentsRosangela Maria Ferreira da Costa e Silva0Ângela Leão Andrade1Erico Tadeu Fraga Freitas2Thalita Marcolan Valverde3Luciano Roni Silva Lara4Darliane Aparecida Martins5Jorge Luis Lopez6Humberto Osório Stumpf7Clésia Cristina Nascentes8Alfredo Miranda de Goes9Rosana Zacarias Domingues10Universidade Estadual de Mato Grosso do SulDepto de Química, ICEB, Universidade Federal de Ouro Preto, UFOPCentro de Microscopia, Universidade Federal de Minas Gerais, UFMGDepto de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, UFMGUniversidade Estadual de Mato Grosso do SulInstituto Federal de Educação, Ciência e Tecnologia do Sul de MinasCentro de Ciências Biológicas e da Natureza, Universidade Federal do Acre, UFACDepto de Química, Universidade Federal de Minas GeraisDepto de Química, Universidade Federal de Minas GeraisDepto de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, UFMGDepto de Química, Universidade Federal de Minas GeraisAbstract Magnetoliposomes containing magnetite, soy lecithin, stigmasterol, and beta-sitosterol of the mean size minor than 160 nm were obtained by a scalable and green process using autoclave and sonication without organic solvents. The formation, size of the liposome, linkage, and encapsulation of the magnetite were evaluated by Cryo-TEM. The stability of magnetoliposomes after storage for 6 months at 4 °C was improved by liposome size, the ability of soy lecithin to preserve the magnetite phase against oxidation, pH, polydispersity index, and zeta potential. The iron oxide phase stability was assessed using no conventional X-ray diffraction (high-resolution transmission electron microscopy), energy loss electron spectroscopy, and selected area electron diffraction) in time zero (fresh sample) and 6 months. The high zeta potential measured for magnetoliposomes, │53│ mV, indicated a low tendency to agglomerate. Lip-Fe3O4@lecithin with concentrations of 0.58 mg mL−1 of liposome showed high cell viability and are potential candidates for drug delivery and hyperthermia treatments in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.https://doi.org/10.1038/s41598-024-82480-xMagnetite stabilitySoy lecithin liposomeEELSCryO-TEMSAED |
| spellingShingle | Rosangela Maria Ferreira da Costa e Silva Ângela Leão Andrade Erico Tadeu Fraga Freitas Thalita Marcolan Valverde Luciano Roni Silva Lara Darliane Aparecida Martins Jorge Luis Lopez Humberto Osório Stumpf Clésia Cristina Nascentes Alfredo Miranda de Goes Rosana Zacarias Domingues Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments Scientific Reports Magnetite stability Soy lecithin liposome EELS CryO-TEM SAED |
| title | Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments |
| title_full | Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments |
| title_fullStr | Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments |
| title_full_unstemmed | Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments |
| title_short | Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments |
| title_sort | formation and stability of green and low cost magnetoliposomes of the soy lecithin stigmasterol and β sitosterol for hyperthermia treatments |
| topic | Magnetite stability Soy lecithin liposome EELS CryO-TEM SAED |
| url | https://doi.org/10.1038/s41598-024-82480-x |
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