Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis
Asthma has been extensively studied in humans and animals, but the molecular mechanisms underlying asthma in Meishan pigs, a breed with distinct genetic and physiological characteristics, remain elusive. Understanding these mechanisms could provide insights into veterinary medicine and human asthma...
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2025-01-01
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| author | Weilong Tu Hongyang Wang Yingying Zhang Ji Huang Yuduan Diao Jieke Zhou Yongsong Tan Xin Li |
| author_facet | Weilong Tu Hongyang Wang Yingying Zhang Ji Huang Yuduan Diao Jieke Zhou Yongsong Tan Xin Li |
| author_sort | Weilong Tu |
| collection | DOAJ |
| description | Asthma has been extensively studied in humans and animals, but the molecular mechanisms underlying asthma in Meishan pigs, a breed with distinct genetic and physiological characteristics, remain elusive. Understanding these mechanisms could provide insights into veterinary medicine and human asthma research. We investigated asthma pathogenesis in Meishan pigs through transcriptomic and metabolomic analyses of blood samples taken during autumn and winter. Asthma in Meishan pigs is related to inflammation, mitochondrial oxidative phosphorylation, and tricarboxylic acid (TCA) cycle disorders. Related genes include <i>CXCL10</i>, <i>CCL8</i>, <i>CCL22</i>, <i>CCL21</i>, <i>OLR1</i>, and <i>ACKR1</i>, while metabolites include succinic acid, riboflavin-5-phosphate, and fumaric acid. Transcriptomic sequencing was performed on panting and normal Meishan pigs, and differentially expressed genes underwent functional enrichment screening. Metabolomic analysis revealed differential metabolites and pathways between groups. Combined analyses indicated that lung inflammation is influenced by genetic, allergenic, and environmental factors disrupting oxidative phosphorylation in lung mitochondria, affecting the TCA cycle. Mitochondrial reactive oxygen species, glutathione S-transferases, arginase 1 and RORC in immune regulation, the Notch pathway, YPEL4 in cell proliferation, and MARCKS in airway mucus secretion play roles in asthma pathogenesis. This study highlights that many cytokines and signaling pathways contribute to asthma. Further studies are needed to elucidate their complex interactions. |
| format | Article |
| id | doaj-art-61f57f8f1a904a26a6636e62f9496788 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-61f57f8f1a904a26a6636e62f94967882025-01-24T13:18:03ZengMDPI AGAnimals2076-26152025-01-0115220010.3390/ani15020200Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics AnalysisWeilong Tu0Hongyang Wang1Yingying Zhang2Ji Huang3Yuduan Diao4Jieke Zhou5Yongsong Tan6Xin Li7Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaAsthma has been extensively studied in humans and animals, but the molecular mechanisms underlying asthma in Meishan pigs, a breed with distinct genetic and physiological characteristics, remain elusive. Understanding these mechanisms could provide insights into veterinary medicine and human asthma research. We investigated asthma pathogenesis in Meishan pigs through transcriptomic and metabolomic analyses of blood samples taken during autumn and winter. Asthma in Meishan pigs is related to inflammation, mitochondrial oxidative phosphorylation, and tricarboxylic acid (TCA) cycle disorders. Related genes include <i>CXCL10</i>, <i>CCL8</i>, <i>CCL22</i>, <i>CCL21</i>, <i>OLR1</i>, and <i>ACKR1</i>, while metabolites include succinic acid, riboflavin-5-phosphate, and fumaric acid. Transcriptomic sequencing was performed on panting and normal Meishan pigs, and differentially expressed genes underwent functional enrichment screening. Metabolomic analysis revealed differential metabolites and pathways between groups. Combined analyses indicated that lung inflammation is influenced by genetic, allergenic, and environmental factors disrupting oxidative phosphorylation in lung mitochondria, affecting the TCA cycle. Mitochondrial reactive oxygen species, glutathione S-transferases, arginase 1 and RORC in immune regulation, the Notch pathway, YPEL4 in cell proliferation, and MARCKS in airway mucus secretion play roles in asthma pathogenesis. This study highlights that many cytokines and signaling pathways contribute to asthma. Further studies are needed to elucidate their complex interactions.https://www.mdpi.com/2076-2615/15/2/200Meishan pigasthmatranscriptomemetabolome |
| spellingShingle | Weilong Tu Hongyang Wang Yingying Zhang Ji Huang Yuduan Diao Jieke Zhou Yongsong Tan Xin Li Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis Animals Meishan pig asthma transcriptome metabolome |
| title | Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis |
| title_full | Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis |
| title_fullStr | Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis |
| title_full_unstemmed | Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis |
| title_short | Investigation of the Molecular Mechanism of Asthma in Meishan Pigs Using Multi-Omics Analysis |
| title_sort | investigation of the molecular mechanism of asthma in meishan pigs using multi omics analysis |
| topic | Meishan pig asthma transcriptome metabolome |
| url | https://www.mdpi.com/2076-2615/15/2/200 |
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