Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice
Introduction. The effects of fatty acids on health vary and depend on the type, amount, and route of consumption. EPA and DHA have a defined role in health, unlike coconut oil. Objective. The aim was to investigate the changes in metabolic regulation and the composition of the culture-dependent micr...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2022/3336941 |
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| author | Beatriz Elina Martínez-Carrillo Talia Mondragón-Velásquez Ninfa Ramírez-Durán José Félix Aguirre-Garrido Roxana Valdés-Ramos Ana Laura Guadarrama-López Arturo Castillo-Cardiel |
| author_facet | Beatriz Elina Martínez-Carrillo Talia Mondragón-Velásquez Ninfa Ramírez-Durán José Félix Aguirre-Garrido Roxana Valdés-Ramos Ana Laura Guadarrama-López Arturo Castillo-Cardiel |
| author_sort | Beatriz Elina Martínez-Carrillo |
| collection | DOAJ |
| description | Introduction. The effects of fatty acids on health vary and depend on the type, amount, and route of consumption. EPA and DHA have a defined role in health, unlike coconut oil. Objective. The aim was to investigate the changes in metabolic regulation and the composition of the culture-dependent microbiota after supplementation with different fatty acids in db/db mice. Material and Methods. We were using 32 8-week-old db/db mice, supplemented for eight weeks with EPA/DHA derived from microalgae as well as coconut oil. The lipid, hormonal profiles, and composition of the culture-dependent microbiota and the phylogenetic analysis based on the 16S rRNA gene sequencing were determined for identification of the intestinal microbiota. Results. Enriched diet with EPA/DHA reduced TNF-α, C-peptide, insulin resistance, resistin, and the plasma atherogenic index, but increased TC, LDL-c, VLDL-c, and TG without changes in HDL-c. Coconut oil raised the HDL-c, GIP, and TNF-α, with TG, insulin resistance, adiponectin, and C-peptide reduced. Conclusion. The most abundant microbial populations were Firmicutes and the least Proteobacteria. EPA/DHA derived from microalgae contributes to improving the systemic inflammatory status, but depressed the diversity of the small intestine microbiota. Coconut oil only decreased the C-peptide, raising TNF-α, with an unfavorable hormonal and lipid profile. |
| format | Article |
| id | doaj-art-60c4a8d9586445b8bc8ba2461e05135d |
| institution | Kabale University |
| issn | 2314-5765 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-60c4a8d9586445b8bc8ba2461e05135d2025-02-03T01:30:38ZengWileyInternational Journal of Food Science2314-57652022-01-01202210.1155/2022/3336941Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db MiceBeatriz Elina Martínez-Carrillo0Talia Mondragón-Velásquez1Ninfa Ramírez-Durán2José Félix Aguirre-Garrido3Roxana Valdés-Ramos4Ana Laura Guadarrama-López5Arturo Castillo-Cardiel6Laboratorio de Investigación en NutriciónLaboratorio de Investigación en NutriciónLaboratorio de Microbiología Médica y AmbientalLaboratorio de Biotecnología AmbientalLaboratorio de Investigación en NutriciónLaboratorio de Investigación en NutriciónDepartamento de Cirugía ExperimentalIntroduction. The effects of fatty acids on health vary and depend on the type, amount, and route of consumption. EPA and DHA have a defined role in health, unlike coconut oil. Objective. The aim was to investigate the changes in metabolic regulation and the composition of the culture-dependent microbiota after supplementation with different fatty acids in db/db mice. Material and Methods. We were using 32 8-week-old db/db mice, supplemented for eight weeks with EPA/DHA derived from microalgae as well as coconut oil. The lipid, hormonal profiles, and composition of the culture-dependent microbiota and the phylogenetic analysis based on the 16S rRNA gene sequencing were determined for identification of the intestinal microbiota. Results. Enriched diet with EPA/DHA reduced TNF-α, C-peptide, insulin resistance, resistin, and the plasma atherogenic index, but increased TC, LDL-c, VLDL-c, and TG without changes in HDL-c. Coconut oil raised the HDL-c, GIP, and TNF-α, with TG, insulin resistance, adiponectin, and C-peptide reduced. Conclusion. The most abundant microbial populations were Firmicutes and the least Proteobacteria. EPA/DHA derived from microalgae contributes to improving the systemic inflammatory status, but depressed the diversity of the small intestine microbiota. Coconut oil only decreased the C-peptide, raising TNF-α, with an unfavorable hormonal and lipid profile.http://dx.doi.org/10.1155/2022/3336941 |
| spellingShingle | Beatriz Elina Martínez-Carrillo Talia Mondragón-Velásquez Ninfa Ramírez-Durán José Félix Aguirre-Garrido Roxana Valdés-Ramos Ana Laura Guadarrama-López Arturo Castillo-Cardiel Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice International Journal of Food Science |
| title | Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice |
| title_full | Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice |
| title_fullStr | Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice |
| title_full_unstemmed | Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice |
| title_short | Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice |
| title_sort | changes in metabolic regulation and the microbiota composition after supplementation with different fatty acids in db db mice |
| url | http://dx.doi.org/10.1155/2022/3336941 |
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