Role of transforming growth factor-β1 in regulating adipocyte progenitors
Abstract Adipose tissue (AT) metabolism involves coordinating various cells and cellular processes to regulate energy storage, release, and overall metabolic homeostasis. Therein, macrophage and its cytokine are important in controlling tissue homeostasis. Among cytokines, the role of transforming g...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-81917-7 |
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| author | Nguyen Quynh Phuong Muhammad Bilal Allah Nawaz Le Duc Anh Memoona Muhammad Rahil Aslam Sana Khalid Tomonobu Kado Yoshiyuki Watanabe Ayumi Nishimura Yoshiko Igarashi Keisuke Okabe Kenichi Hirabayashi Seiji Yamamoto Takashi Nakagawa Hisashi Mori Isao Usui Shiho Fujisaka Ryuji Hayashi Kazuyuki Tobe |
| author_facet | Nguyen Quynh Phuong Muhammad Bilal Allah Nawaz Le Duc Anh Memoona Muhammad Rahil Aslam Sana Khalid Tomonobu Kado Yoshiyuki Watanabe Ayumi Nishimura Yoshiko Igarashi Keisuke Okabe Kenichi Hirabayashi Seiji Yamamoto Takashi Nakagawa Hisashi Mori Isao Usui Shiho Fujisaka Ryuji Hayashi Kazuyuki Tobe |
| author_sort | Nguyen Quynh Phuong |
| collection | DOAJ |
| description | Abstract Adipose tissue (AT) metabolism involves coordinating various cells and cellular processes to regulate energy storage, release, and overall metabolic homeostasis. Therein, macrophage and its cytokine are important in controlling tissue homeostasis. Among cytokines, the role of transforming growth factor-β1 (Tgf-β1), a cytokine abundantly expressed in CD206+ M2-like macrophage and correlated with the expansion of AT and fibrosis, in AT metabolism, remains unknown. We used CD206CreERT2; Tgf-β1f/f mouse model in which the Tgf-β1 gene was conditionally deleted in CD206+ M2-like macrophages followed by tamoxifen administration, to investigate the role of the Tgf-β1 gene in glucose and insulin metabolism. Our data demonstrated that lack of CD206+ M2-like macrophages derived Tgf-β1 gene improved glucose metabolism and insulin sensitivity by enhancing adipogenesis via hyperplasia. The Tgf-β1 gene, specifically from CD206+ M2-like macrophages, deletion stimulated APs’ proliferation and differentiation, leading to the generation of smaller mature adipocytes, therefore enhancing insulin sensitivity and improving glucose metabolism under normal chow conditions. Our study brings a new perspective that Tgf-β1 gene deletion specific from CD206+ M2-like macrophage promotes adipocyte hyperplasia, improving glucose homeostasis and insulin sensitivity in the lean state. |
| format | Article |
| id | doaj-art-603fbaca669442839e624dd0e110c331 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-603fbaca669442839e624dd0e110c3312025-01-19T12:21:17ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-81917-7Role of transforming growth factor-β1 in regulating adipocyte progenitorsNguyen Quynh Phuong0Muhammad Bilal1Allah Nawaz2Le Duc Anh3Memoona4Muhammad Rahil Aslam5Sana Khalid6Tomonobu Kado7Yoshiyuki Watanabe8Ayumi Nishimura9Yoshiko Igarashi10Keisuke Okabe11Kenichi Hirabayashi12Seiji Yamamoto13Takashi Nakagawa14Hisashi Mori15Isao Usui16Shiho Fujisaka17Ryuji Hayashi18Kazuyuki Tobe19First Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaDepartment of Molecular Neuroscience, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaToyama University Hospital, Center for Clinical and Translational ResearchDepartment of Diagnostic Pathology, Faculty of Medicine, University of ToyamaDepartment of Pathology, Faculty of Medicine, University of ToyamaDepartment of Molecular and Medical Pharmacology, Faculty of Medicine, University of ToyamaDepartment of Molecular Neuroscience, Faculty of Medicine, University of ToyamaDepartment of Endocrinology and Metabolism, Dokkyo Medical UniversityFirst Department of Internal Medicine, Faculty of Medicine, University of ToyamaClinical Oncology, Faculty of Medicine, University of ToyamaResearch Center for Pre-Disease Science, Faculty of Education and Research Promotion, University of ToyamaAbstract Adipose tissue (AT) metabolism involves coordinating various cells and cellular processes to regulate energy storage, release, and overall metabolic homeostasis. Therein, macrophage and its cytokine are important in controlling tissue homeostasis. Among cytokines, the role of transforming growth factor-β1 (Tgf-β1), a cytokine abundantly expressed in CD206+ M2-like macrophage and correlated with the expansion of AT and fibrosis, in AT metabolism, remains unknown. We used CD206CreERT2; Tgf-β1f/f mouse model in which the Tgf-β1 gene was conditionally deleted in CD206+ M2-like macrophages followed by tamoxifen administration, to investigate the role of the Tgf-β1 gene in glucose and insulin metabolism. Our data demonstrated that lack of CD206+ M2-like macrophages derived Tgf-β1 gene improved glucose metabolism and insulin sensitivity by enhancing adipogenesis via hyperplasia. The Tgf-β1 gene, specifically from CD206+ M2-like macrophages, deletion stimulated APs’ proliferation and differentiation, leading to the generation of smaller mature adipocytes, therefore enhancing insulin sensitivity and improving glucose metabolism under normal chow conditions. Our study brings a new perspective that Tgf-β1 gene deletion specific from CD206+ M2-like macrophage promotes adipocyte hyperplasia, improving glucose homeostasis and insulin sensitivity in the lean state.https://doi.org/10.1038/s41598-024-81917-7Tgf-β1CD206+ M2-like macrophageAdipocyte progenitorsHyperplasiaAdipogenesis |
| spellingShingle | Nguyen Quynh Phuong Muhammad Bilal Allah Nawaz Le Duc Anh Memoona Muhammad Rahil Aslam Sana Khalid Tomonobu Kado Yoshiyuki Watanabe Ayumi Nishimura Yoshiko Igarashi Keisuke Okabe Kenichi Hirabayashi Seiji Yamamoto Takashi Nakagawa Hisashi Mori Isao Usui Shiho Fujisaka Ryuji Hayashi Kazuyuki Tobe Role of transforming growth factor-β1 in regulating adipocyte progenitors Scientific Reports Tgf-β1 CD206+ M2-like macrophage Adipocyte progenitors Hyperplasia Adipogenesis |
| title | Role of transforming growth factor-β1 in regulating adipocyte progenitors |
| title_full | Role of transforming growth factor-β1 in regulating adipocyte progenitors |
| title_fullStr | Role of transforming growth factor-β1 in regulating adipocyte progenitors |
| title_full_unstemmed | Role of transforming growth factor-β1 in regulating adipocyte progenitors |
| title_short | Role of transforming growth factor-β1 in regulating adipocyte progenitors |
| title_sort | role of transforming growth factor β1 in regulating adipocyte progenitors |
| topic | Tgf-β1 CD206+ M2-like macrophage Adipocyte progenitors Hyperplasia Adipogenesis |
| url | https://doi.org/10.1038/s41598-024-81917-7 |
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