Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal
Flavonoids have achieved widespread importance in pharmaceutical, food, and cosmetics industries. Furthermore, modification of these naturally occurring flavonoids to structurally diverse compounds through whole cell biotransformation with enhanced biological activities has numerous biotechnological...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/9948738 |
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| author | Lasata Shrestha Bishnu P. Marasini Suman Prakash Pradhan Rajib Kumar Shrestha Suraj Shrestha Kamal Prasad Regmi Bishnu Prasad Pandey |
| author_facet | Lasata Shrestha Bishnu P. Marasini Suman Prakash Pradhan Rajib Kumar Shrestha Suraj Shrestha Kamal Prasad Regmi Bishnu Prasad Pandey |
| author_sort | Lasata Shrestha |
| collection | DOAJ |
| description | Flavonoids have achieved widespread importance in pharmaceutical, food, and cosmetics industries. Furthermore, modification of these naturally occurring flavonoids to structurally diverse compounds through whole cell biotransformation with enhanced biological activities has numerous biotechnological applications. The present study investigated the biotransformation potential of Streptomyces species isolated from a high-altitude-soil sample towards selected flavonoid molecules. The biotransformed metabolites were confirmed by comparing the HPLC chromatogram with authentic compounds and LC-MS/MS analysis. Of these isolates, Streptomyces species G-18 (Accession number: MW663767.1) catalyzed isoflavone molecules daidzein and genistein to produce hydroxylated products at 24 h of reaction condition in a whole cell system. The hydroxylation of daidzein (4′,7-dihydroxyisoflavone) was confirmed at 3′-position of the B ring to produce 3′,4′,7-trihydroxyisoflavone. In addition, Streptomyces species G-14 (Accession number: MW663770.1) and Streptomyces species S4L (Accession number: MW663769.1) also revealed the transformation of daidzein (4′,7-dihydroxyisoflavone) to hydroxy daidzein at a distinct position than that of G-18 isolates, whereas thee Streptomyces species S4L reaction mixture with naringenin as a substrate also revealed the hydroxylated product. Our results demonstrated that microorganisms isolated from different ecological niches have broad application. |
| format | Article |
| id | doaj-art-5ffa42d77d0e43bf8c22114a56ee4456 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-5ffa42d77d0e43bf8c22114a56ee44562025-02-03T07:23:29ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/99487389948738Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of NepalLasata Shrestha0Bishnu P. Marasini1Suman Prakash Pradhan2Rajib Kumar Shrestha3Suraj Shrestha4Kamal Prasad Regmi5Bishnu Prasad Pandey6Department of Chemical Science and Engineering, Kathmandu University, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Environmental Science and Engineering, Kathmandu University, Kathmandu, NepalDepartment of Chemical Science and Engineering, Kathmandu University, Kathmandu, NepalDepartment of Food Technology and Quality Control, Ministry of Agriculture and Livestock Development, Babarmahal, Kathmandu, NepalDepartment of Food Technology and Quality Control, Ministry of Agriculture and Livestock Development, Babarmahal, Kathmandu, NepalDepartment of Chemical Science and Engineering, Kathmandu University, Kathmandu, NepalFlavonoids have achieved widespread importance in pharmaceutical, food, and cosmetics industries. Furthermore, modification of these naturally occurring flavonoids to structurally diverse compounds through whole cell biotransformation with enhanced biological activities has numerous biotechnological applications. The present study investigated the biotransformation potential of Streptomyces species isolated from a high-altitude-soil sample towards selected flavonoid molecules. The biotransformed metabolites were confirmed by comparing the HPLC chromatogram with authentic compounds and LC-MS/MS analysis. Of these isolates, Streptomyces species G-18 (Accession number: MW663767.1) catalyzed isoflavone molecules daidzein and genistein to produce hydroxylated products at 24 h of reaction condition in a whole cell system. The hydroxylation of daidzein (4′,7-dihydroxyisoflavone) was confirmed at 3′-position of the B ring to produce 3′,4′,7-trihydroxyisoflavone. In addition, Streptomyces species G-14 (Accession number: MW663770.1) and Streptomyces species S4L (Accession number: MW663769.1) also revealed the transformation of daidzein (4′,7-dihydroxyisoflavone) to hydroxy daidzein at a distinct position than that of G-18 isolates, whereas thee Streptomyces species S4L reaction mixture with naringenin as a substrate also revealed the hydroxylated product. Our results demonstrated that microorganisms isolated from different ecological niches have broad application.http://dx.doi.org/10.1155/2021/9948738 |
| spellingShingle | Lasata Shrestha Bishnu P. Marasini Suman Prakash Pradhan Rajib Kumar Shrestha Suraj Shrestha Kamal Prasad Regmi Bishnu Prasad Pandey Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal International Journal of Microbiology |
| title | Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal |
| title_full | Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal |
| title_fullStr | Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal |
| title_full_unstemmed | Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal |
| title_short | Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal |
| title_sort | biotransformation of daidzein genistein and naringenin by streptomyces species isolated from high altitude soil of nepal |
| url | http://dx.doi.org/10.1155/2021/9948738 |
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