Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis
Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/4758930 |
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| author | Ignacio Muñoz-Criado Jose Meseguer-Ripolles Maravillas Mellado-López Ana Alastrue-Agudo Richard J Griffeth Jerónimo Forteza-Vila Ramón Cugat Montserrat García Victoria Moreno-Manzano |
| author_facet | Ignacio Muñoz-Criado Jose Meseguer-Ripolles Maravillas Mellado-López Ana Alastrue-Agudo Richard J Griffeth Jerónimo Forteza-Vila Ramón Cugat Montserrat García Victoria Moreno-Manzano |
| author_sort | Ignacio Muñoz-Criado |
| collection | DOAJ |
| description | Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution. When infrapatellar- and suprapatellar-derived stromal vascular fractions (SVF) were compared, a significantly higher CD105 (+) population was found in the suprapatellar fat. In addition, the suprapatellar SVF exhibited increased numbers of colony formation units and a higher population doubling in culture compared to the infrapatellar fraction. Both the suprapatellar- and infrapatellar-derived ASC were differentiated in vitro into mature adipocytes, osteocytes, and chondrocytes. However, the suprapatellar-derived ASC showed higher osteogenic and chondrogenic efficiency. Suprapatellar-derived ASC transplantation in a severe OA mouse model significantly diminished the OA-associated knee inflammation and cartilage degenerative grade, significantly increasing the production of glycosaminoglycan and inducing endogenous chondrogenesis in comparison with the control group. Overall, suprapatellar-derived ASC offer a potential autologous regenerative treatment for patients with multiple degenerative OA. |
| format | Article |
| id | doaj-art-5eb04d90faa2464e95c6e507edef5d73 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5eb04d90faa2464e95c6e507edef5d732025-02-03T05:48:16ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/47589304758930Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe OsteoarthritisIgnacio Muñoz-Criado0Jose Meseguer-Ripolles1Maravillas Mellado-López2Ana Alastrue-Agudo3Richard J Griffeth4Jerónimo Forteza-Vila5Ramón Cugat6Montserrat García7Victoria Moreno-Manzano8Unidad de Traumatología, Hospital Casa de la Salud, Valencia, SpainNeuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, SpainNeuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, SpainNeuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, SpainNeuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, SpainMolecular Pathology and Translational Research in Oncology, Unidad Mixta Universidad Católica de Valencia y Centro de Investigación Príncipe Felipe, Valencia, SpainUnidad de Artroscopia y Unidad de Traumatología del Hospital Quiron, Barcelona, SpainUnidad de Artroscopia y Unidad de Traumatología del Hospital Quiron, Barcelona, SpainNeuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, SpainCartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution. When infrapatellar- and suprapatellar-derived stromal vascular fractions (SVF) were compared, a significantly higher CD105 (+) population was found in the suprapatellar fat. In addition, the suprapatellar SVF exhibited increased numbers of colony formation units and a higher population doubling in culture compared to the infrapatellar fraction. Both the suprapatellar- and infrapatellar-derived ASC were differentiated in vitro into mature adipocytes, osteocytes, and chondrocytes. However, the suprapatellar-derived ASC showed higher osteogenic and chondrogenic efficiency. Suprapatellar-derived ASC transplantation in a severe OA mouse model significantly diminished the OA-associated knee inflammation and cartilage degenerative grade, significantly increasing the production of glycosaminoglycan and inducing endogenous chondrogenesis in comparison with the control group. Overall, suprapatellar-derived ASC offer a potential autologous regenerative treatment for patients with multiple degenerative OA.http://dx.doi.org/10.1155/2017/4758930 |
| spellingShingle | Ignacio Muñoz-Criado Jose Meseguer-Ripolles Maravillas Mellado-López Ana Alastrue-Agudo Richard J Griffeth Jerónimo Forteza-Vila Ramón Cugat Montserrat García Victoria Moreno-Manzano Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis Stem Cells International |
| title | Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis |
| title_full | Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis |
| title_fullStr | Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis |
| title_full_unstemmed | Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis |
| title_short | Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis |
| title_sort | human suprapatellar fat pad derived mesenchymal stem cells induce chondrogenesis and cartilage repair in a model of severe osteoarthritis |
| url | http://dx.doi.org/10.1155/2017/4758930 |
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