Sensitive Spectrophotometric Methods for the Determination of Gatifloxacin in Pharmaceuticals Using Bromate-Bromide, Methylene Blue and Rhodamine-B as Reagents
Two new simple, precise, rapid and extraction-free spectrophotometric methods are proposed for the determination of gatifloxacin(GTF) using bromate-bromide mixture and two dyes, methylene blue and rhodamine B, as reagents. Spectrophotometric methods entail the addition of a known excess of bromate-b...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2007-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2007/374837 |
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| Summary: | Two new simple, precise, rapid and extraction-free spectrophotometric methods are proposed for the determination of gatifloxacin(GTF) using bromate-bromide mixture and two dyes, methylene blue and rhodamine B, as reagents. Spectrophotometric methods entail the addition of a known excess of bromate-bromide mixture to GTF in hydrochloric acid medium followed by determination of residual bromine by reacting with a fixed amount of either methylene blue and measuring the absorbance at 665 nm (Method A) or rhodamine B and measuring the absorbance at 555 nm (Method B). Beer᾽s law is obeyed in the ranges, 0.5-5.0 and 0.2-1.5 μg mL-1 for method A and method B, respectively. The apparent molar absorptivities are calculated to be 5.6×104 and 9.6×104 L mol-1cm-1 for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0071 and 0.0042 μg cm-2. The methods were successfully applied to the assay of GTF in pharmaceutical formulations with satisfactory results. |
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| ISSN: | 0973-4945 2090-9810 |