A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice
Anesthetic isoflurane (ISO) has immunomodulatory effects. In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in the murine lung and isolated neutrophils. At 1 and 6 hrs after ZY administration intraperitoneal...
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/479628 |
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| author | Hui Wang Jing Fan Nan-lin Li Jun-tang Li Shi-fang Yuan Jun Yi Ling Wang Jiang-hao Chen Yong-gang Lv Qing Yao Ting Wang Yu-cai Wang Rui Ling |
| author_facet | Hui Wang Jing Fan Nan-lin Li Jun-tang Li Shi-fang Yuan Jun Yi Ling Wang Jiang-hao Chen Yong-gang Lv Qing Yao Ting Wang Yu-cai Wang Rui Ling |
| author_sort | Hui Wang |
| collection | DOAJ |
| description | Anesthetic isoflurane (ISO) has immunomodulatory effects. In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in the murine lung and isolated neutrophils. At 1 and 6 hrs after ZY administration intraperitoneally, ISO was inhaled for 1 hr, and 24 hrs later, lung inflammation and injury were assessed. We found that ISO improved the survival rate of mice and mitigated lung injury as characterized by the histopathology, wet-to-dry weight ratio, protein leakage, and lung function index. ISO significantly attenuated ZY-induced lung neutrophil recruitment and inflammation. This was suggested by the downregulation of (a) endothelial adhesion molecule expression and myeloperoxidase (MPO) activity in lung tissue and polymorphonuclear neutrophils (b) chemokines, and (c) proinflammatory cytokines in BALF. Furthermore, ZY-induced nuclear translocation and DNA-binding activity of NF-κB p65 were also reduced by ISO. ISO treatment inhibited iNOS expression and activity, as well as subsequent nitric oxide generation. Consistent with these in vivo observations, in vitro studies confirmed that ISO blocked NF-κB and iNOS activation in primary mouse neutrophils challenged by ZY. These results provide evidence that 0.7% ISO ameliorates inflammatory responses in ZY-treated mouse lung and primary neutrophils. |
| format | Article |
| id | doaj-art-5d226f9a7c364833b89b67a381f9cf03 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5d226f9a7c364833b89b67a381f9cf032025-02-03T05:50:26ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/479628479628A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in MiceHui Wang0Jing Fan1Nan-lin Li2Jun-tang Li3Shi-fang Yuan4Jun Yi5Ling Wang6Jiang-hao Chen7Yong-gang Lv8Qing Yao9Ting Wang10Yu-cai Wang11Rui Ling12Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaAnesthetic isoflurane (ISO) has immunomodulatory effects. In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in the murine lung and isolated neutrophils. At 1 and 6 hrs after ZY administration intraperitoneally, ISO was inhaled for 1 hr, and 24 hrs later, lung inflammation and injury were assessed. We found that ISO improved the survival rate of mice and mitigated lung injury as characterized by the histopathology, wet-to-dry weight ratio, protein leakage, and lung function index. ISO significantly attenuated ZY-induced lung neutrophil recruitment and inflammation. This was suggested by the downregulation of (a) endothelial adhesion molecule expression and myeloperoxidase (MPO) activity in lung tissue and polymorphonuclear neutrophils (b) chemokines, and (c) proinflammatory cytokines in BALF. Furthermore, ZY-induced nuclear translocation and DNA-binding activity of NF-κB p65 were also reduced by ISO. ISO treatment inhibited iNOS expression and activity, as well as subsequent nitric oxide generation. Consistent with these in vivo observations, in vitro studies confirmed that ISO blocked NF-κB and iNOS activation in primary mouse neutrophils challenged by ZY. These results provide evidence that 0.7% ISO ameliorates inflammatory responses in ZY-treated mouse lung and primary neutrophils.http://dx.doi.org/10.1155/2013/479628 |
| spellingShingle | Hui Wang Jing Fan Nan-lin Li Jun-tang Li Shi-fang Yuan Jun Yi Ling Wang Jiang-hao Chen Yong-gang Lv Qing Yao Ting Wang Yu-cai Wang Rui Ling A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice Mediators of Inflammation |
| title | A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice |
| title_full | A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice |
| title_fullStr | A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice |
| title_full_unstemmed | A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice |
| title_short | A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice |
| title_sort | subanesthetic dose of isoflurane during postconditioning ameliorates zymosan induced neutrophil inflammation lung injury and mortality in mice |
| url | http://dx.doi.org/10.1155/2013/479628 |
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