Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i>
Superoxide dismutase (SOD) enzymes are essential for reducing oxidative damage resulting from overabundant reactive oxygen species under abiotic stress. While the <i>SOD</i> gene family has been extensively studied in many species, research focusing on <i>Cymbidium</i> specie...
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2025-01-01
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| author | Ruyi Li Songkun Lin Yin Yan Yuming Chen Linying Wang Yuzhen Zhou Shuling Tang Ning Liu |
| author_facet | Ruyi Li Songkun Lin Yin Yan Yuming Chen Linying Wang Yuzhen Zhou Shuling Tang Ning Liu |
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| description | Superoxide dismutase (SOD) enzymes are essential for reducing oxidative damage resulting from overabundant reactive oxygen species under abiotic stress. While the <i>SOD</i> gene family has been extensively studied in many species, research focusing on <i>Cymbidium</i> species remains limited. In this study, a comprehensive analysis of the <i>SOD</i> gene family in three <i>Cymbidium</i> genomes was conducted. A total of 23 <i>SOD</i> genes were identified, with nine <i>SOD</i>s in <i>C. sinense</i>, eight in <i>C. ensifolium</i>, and six in <i>C. goeringii</i>. These <i>SOD</i> genes were categorized into three clades: Cu/Zn-SOD, Fe-SOD, and Mn-SOD, with the Cu/Zn-SOD being the most abundant in these three types. This classification was supported by analyses of conserved domains, motifs, and phylogenetic relationships. <i>Cis</i>-element prediction showed that stress-responsive elements were identified in most <i>SOD</i>s. Transcriptomic data revealed that seven <i>CsSOD</i>s exhibited a border expression in all sequenced tissues, while two exhibited undetectable expression levels. Further qRT-PCR analysis showed that all <i>CsSOD</i>s were upregulated under salt stress, with some exhibiting significant changes in expression. These findings all highlight the crucial role of <i>CsSOD</i>s in the salt stress response and provide valuable insights for further breeding salt-tolerance varieties of <i>C. sinense</i>. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Horticulturae |
| spelling | doaj-art-5c80ff46bc0c4a4a8c655db06d00a8472025-01-24T13:34:45ZengMDPI AGHorticulturae2311-75242025-01-011119510.3390/horticulturae11010095Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i>Ruyi Li0Songkun Lin1Yin Yan2Yuming Chen3Linying Wang4Yuzhen Zhou5Shuling Tang6Ning Liu7Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSuperoxide dismutase (SOD) enzymes are essential for reducing oxidative damage resulting from overabundant reactive oxygen species under abiotic stress. While the <i>SOD</i> gene family has been extensively studied in many species, research focusing on <i>Cymbidium</i> species remains limited. In this study, a comprehensive analysis of the <i>SOD</i> gene family in three <i>Cymbidium</i> genomes was conducted. A total of 23 <i>SOD</i> genes were identified, with nine <i>SOD</i>s in <i>C. sinense</i>, eight in <i>C. ensifolium</i>, and six in <i>C. goeringii</i>. These <i>SOD</i> genes were categorized into three clades: Cu/Zn-SOD, Fe-SOD, and Mn-SOD, with the Cu/Zn-SOD being the most abundant in these three types. This classification was supported by analyses of conserved domains, motifs, and phylogenetic relationships. <i>Cis</i>-element prediction showed that stress-responsive elements were identified in most <i>SOD</i>s. Transcriptomic data revealed that seven <i>CsSOD</i>s exhibited a border expression in all sequenced tissues, while two exhibited undetectable expression levels. Further qRT-PCR analysis showed that all <i>CsSOD</i>s were upregulated under salt stress, with some exhibiting significant changes in expression. These findings all highlight the crucial role of <i>CsSOD</i>s in the salt stress response and provide valuable insights for further breeding salt-tolerance varieties of <i>C. sinense</i>.https://www.mdpi.com/2311-7524/11/1/95orchid<i>Cymbidium sinense</i><i>SOD</i> gene familysalt stress |
| spellingShingle | Ruyi Li Songkun Lin Yin Yan Yuming Chen Linying Wang Yuzhen Zhou Shuling Tang Ning Liu Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> Horticulturae orchid <i>Cymbidium sinense</i> <i>SOD</i> gene family salt stress |
| title | Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> |
| title_full | Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> |
| title_fullStr | Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> |
| title_full_unstemmed | Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> |
| title_short | Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i> |
| title_sort | genome wide identification of superoxide dismutase i sod i gene family in i cymbidium i species and functional analysis of i cssod i s under salt stress in i cymbidium sinense i |
| topic | orchid <i>Cymbidium sinense</i> <i>SOD</i> gene family salt stress |
| url | https://www.mdpi.com/2311-7524/11/1/95 |
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