Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet
In rodents, the most adequate model of human diet-induced obesity is obesity caused by the consumption of a sweet-fat diet (SFD), which causes more pronounced adiposity in females than in males. The aim of this work was to determine the sex-associated effect of SFD on the expression of genes related...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2844 |
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| author | N. M. Bazhan T. V. Iakovleva A. D. Dubinina E. N. Makarova |
| author_facet | N. M. Bazhan T. V. Iakovleva A. D. Dubinina E. N. Makarova |
| author_sort | N. M. Bazhan |
| collection | DOAJ |
| description | In rodents, the most adequate model of human diet-induced obesity is obesity caused by the consumption of a sweet-fat diet (SFD), which causes more pronounced adiposity in females than in males. The aim of this work was to determine the sex-associated effect of SFD on the expression of genes related to carbohydrate-lipid metabolism in adult mice. For 10 weeks, male and female С57Bl mice were fed a standard laboratory chow (Control group) or a diet, which consisted of laboratory chow supplemented with sweet cookies, sunflower seeds and lard (SFD group). Weights of body, liver and fat depots, blood concentrations of hormones and metabolites, liver fat, and mRNA levels of genes involved in regulation of energy metabolism in the liver, perigonadal and subcutaneous white adipose tissue (pgWAT, scWAT) and brown adipose tissue (BAT) were measured. SFD increased body weight and insulin resistance in mice of both sexes. Female mice that consumed SFD (SFD females) had a greater increase in adiposity than SFD males. SFD females showed a decreased expression of genes related to lipogenesis (Lpl) and glucose metabolism (G6pc, Pklr) in liver, as well as lipogenesis (Lpl, Slca4) and lipolysis (Lipe) in pgWAT, suggesting reduced energy expenditure. In contrast, SFD males showed increased lean mass gain, plasma insulin and FGF21 levels, expressions of Cpt1α gene in pgWAT and scWAT and Pklr gene in liver, suggesting enhanced lipid and glucose oxidation in these organs. Thus, in mice, there are sex-dependent differences in adaptation to SFD at the transcriptional level, which can help to explain higher adiposity in females under SFD consumtion. |
| format | Article |
| id | doaj-art-5c2924bc4bcf4306b6dbce6dab5f6876 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-5c2924bc4bcf4306b6dbce6dab5f68762025-02-01T09:58:09ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-12-0124884485210.18699/VJ20.6821114Impact of sex on the adaptation of adult mice to long consumption of sweet-fat dietN. M. Bazhan0T. V. Iakovleva1A. D. Dubinina2E. N. Makarova3Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesIn rodents, the most adequate model of human diet-induced obesity is obesity caused by the consumption of a sweet-fat diet (SFD), which causes more pronounced adiposity in females than in males. The aim of this work was to determine the sex-associated effect of SFD on the expression of genes related to carbohydrate-lipid metabolism in adult mice. For 10 weeks, male and female С57Bl mice were fed a standard laboratory chow (Control group) or a diet, which consisted of laboratory chow supplemented with sweet cookies, sunflower seeds and lard (SFD group). Weights of body, liver and fat depots, blood concentrations of hormones and metabolites, liver fat, and mRNA levels of genes involved in regulation of energy metabolism in the liver, perigonadal and subcutaneous white adipose tissue (pgWAT, scWAT) and brown adipose tissue (BAT) were measured. SFD increased body weight and insulin resistance in mice of both sexes. Female mice that consumed SFD (SFD females) had a greater increase in adiposity than SFD males. SFD females showed a decreased expression of genes related to lipogenesis (Lpl) and glucose metabolism (G6pc, Pklr) in liver, as well as lipogenesis (Lpl, Slca4) and lipolysis (Lipe) in pgWAT, suggesting reduced energy expenditure. In contrast, SFD males showed increased lean mass gain, plasma insulin and FGF21 levels, expressions of Cpt1α gene in pgWAT and scWAT and Pklr gene in liver, suggesting enhanced lipid and glucose oxidation in these organs. Thus, in mice, there are sex-dependent differences in adaptation to SFD at the transcriptional level, which can help to explain higher adiposity in females under SFD consumtion.https://vavilov.elpub.ru/jour/article/view/2844c57bl/6j micesweet-fat dietadipositysex differencesliveradipose tissuefgf21, insulingene expression |
| spellingShingle | N. M. Bazhan T. V. Iakovleva A. D. Dubinina E. N. Makarova Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet Вавиловский журнал генетики и селекции c57bl/6j mice sweet-fat diet adiposity sex differences liver adipose tissue fgf21, insulin gene expression |
| title | Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet |
| title_full | Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet |
| title_fullStr | Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet |
| title_full_unstemmed | Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet |
| title_short | Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet |
| title_sort | impact of sex on the adaptation of adult mice to long consumption of sweet fat diet |
| topic | c57bl/6j mice sweet-fat diet adiposity sex differences liver adipose tissue fgf21, insulin gene expression |
| url | https://vavilov.elpub.ru/jour/article/view/2844 |
| work_keys_str_mv | AT nmbazhan impactofsexontheadaptationofadultmicetolongconsumptionofsweetfatdiet AT tviakovleva impactofsexontheadaptationofadultmicetolongconsumptionofsweetfatdiet AT addubinina impactofsexontheadaptationofadultmicetolongconsumptionofsweetfatdiet AT enmakarova impactofsexontheadaptationofadultmicetolongconsumptionofsweetfatdiet |