Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue
Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in a closed system device, has been successfully used in aesthetic medicine as well as in ort...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5901479 |
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| author | Sara Nava Valeria Sordi Luisa Pascucci Carlo Tremolada Emilio Ciusani Offer Zeira Moris Cadei Gianni Soldati Augusto Pessina Eugenio Parati Mark Slevin Giulio Alessandri |
| author_facet | Sara Nava Valeria Sordi Luisa Pascucci Carlo Tremolada Emilio Ciusani Offer Zeira Moris Cadei Gianni Soldati Augusto Pessina Eugenio Parati Mark Slevin Giulio Alessandri |
| author_sort | Sara Nava |
| collection | DOAJ |
| description | Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in a closed system device, has been successfully used in aesthetic medicine as well as in orthopedic and general surgery. Interestingly, in orthopedic diseases, this ready-to-use adipose tissue cell derivative seems to have a prolonged time efficacy even upon a single shot injection into osteoarthritic tissues. Here, we investigated the long-term survival and content of MSCs as well the anti-inflammatory activity of LP and its derived MFAT in vitro, with the aim to better understand a possible in vivo mechanism of action. MFAT and LP specimens from 17 human donors were investigated side by side. During a long-term culture in serum-free medium, we found that the total cell number as well the MSC content in MFAT decreased more slowly if compared to those from LP specimens. The analysis of cytokines and growth factors secreted into the conditioned medium (CM) was similar in MFAT and LP during the first week of culture, but the total amount of cytokines secreted by LP decreased much more rapidly than those produced by MFAT during prolonged culture (up to 28 days). Similarly, the addition of MFAT-CM recovered at early (3-7 days) and late stage (14-28 days) of culture strongly inhibited inflammatory function of U937 monocyte cell line, whereas the anti-inflammatory activity of LP-CM was drastically reduced after only 7 days of culture. We conclude that MFAT is an effective preparation with a long-lasting anti-inflammatory activity probably mediated by a long-term survival of their MSC content that releases a combination of cytokines that affect several mechanisms involved in inflammation processes. |
| format | Article |
| id | doaj-art-5b5dd770871a46c8beb4f4b0f7b85fe9 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5b5dd770871a46c8beb4f4b0f7b85fe92025-02-03T05:54:02ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/59014795901479Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose TissueSara Nava0Valeria Sordi1Luisa Pascucci2Carlo Tremolada3Emilio Ciusani4Offer Zeira5Moris Cadei6Gianni Soldati7Augusto Pessina8Eugenio Parati9Mark Slevin10Giulio Alessandri11Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, Fondazione IRCCS Neurological Institute C. Besta, Milan, ItalyDiabetes Research Institute, IRCCS San Raffaele Scientific Institute, 20132 Milan, ItalyDepartment of Veterinary Medicine, University of Perugia, Perugia, ItalyImage Institute, Milan, ItalyLaboratory of Clinical Pathology and Neurogenetic Medicine, Fondazione IRCCS Neurological Institute C. Besta, Milan, ItalySan Michele Veterinary Hospital, Tavezzano con Villavesco, Lodi, ItalySection of Pathological Anatomy DMMT, University of Brescia, Brescia, ItalySwiss Stem Cell Foundation, In Pasquée 23, Gentilino, CH-6925 Lugano, SwitzerlandDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyCellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, Fondazione IRCCS Neurological Institute C. Besta, Milan, ItalySchool of Healthcare Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UKCellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, Fondazione IRCCS Neurological Institute C. Besta, Milan, ItalyOver the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in a closed system device, has been successfully used in aesthetic medicine as well as in orthopedic and general surgery. Interestingly, in orthopedic diseases, this ready-to-use adipose tissue cell derivative seems to have a prolonged time efficacy even upon a single shot injection into osteoarthritic tissues. Here, we investigated the long-term survival and content of MSCs as well the anti-inflammatory activity of LP and its derived MFAT in vitro, with the aim to better understand a possible in vivo mechanism of action. MFAT and LP specimens from 17 human donors were investigated side by side. During a long-term culture in serum-free medium, we found that the total cell number as well the MSC content in MFAT decreased more slowly if compared to those from LP specimens. The analysis of cytokines and growth factors secreted into the conditioned medium (CM) was similar in MFAT and LP during the first week of culture, but the total amount of cytokines secreted by LP decreased much more rapidly than those produced by MFAT during prolonged culture (up to 28 days). Similarly, the addition of MFAT-CM recovered at early (3-7 days) and late stage (14-28 days) of culture strongly inhibited inflammatory function of U937 monocyte cell line, whereas the anti-inflammatory activity of LP-CM was drastically reduced after only 7 days of culture. We conclude that MFAT is an effective preparation with a long-lasting anti-inflammatory activity probably mediated by a long-term survival of their MSC content that releases a combination of cytokines that affect several mechanisms involved in inflammation processes.http://dx.doi.org/10.1155/2019/5901479 |
| spellingShingle | Sara Nava Valeria Sordi Luisa Pascucci Carlo Tremolada Emilio Ciusani Offer Zeira Moris Cadei Gianni Soldati Augusto Pessina Eugenio Parati Mark Slevin Giulio Alessandri Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue Stem Cells International |
| title | Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue |
| title_full | Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue |
| title_fullStr | Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue |
| title_full_unstemmed | Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue |
| title_short | Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue |
| title_sort | long lasting anti inflammatory activity of human microfragmented adipose tissue |
| url | http://dx.doi.org/10.1155/2019/5901479 |
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