Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis
This work’s goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized usin...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/5871043 |
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| author | Juliana Seger Link Silvana Maria Alban Carlos Ricardo Soccol Gilberto Vinicius Melo Pereira Vanete Thomaz Soccol |
| author_facet | Juliana Seger Link Silvana Maria Alban Carlos Ricardo Soccol Gilberto Vinicius Melo Pereira Vanete Thomaz Soccol |
| author_sort | Juliana Seger Link |
| collection | DOAJ |
| description | This work’s goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund’s adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides’ sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides’ MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis. |
| format | Article |
| id | doaj-art-59d745b3d0a2410f8ba8759a76c3beff |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-59d745b3d0a2410f8ba8759a76c3beff2025-02-03T01:32:56ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/58710435871043Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis DiagnosisJuliana Seger Link0Silvana Maria Alban1Carlos Ricardo Soccol2Gilberto Vinicius Melo Pereira3Vanete Thomaz Soccol4Basic Pathology Department, Federal University of Paraná, Curitiba, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, BrazilBasic Pathology Department, Federal University of Paraná, Curitiba, PR, BrazilThis work’s goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund’s adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides’ sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides’ MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis.http://dx.doi.org/10.1155/2017/5871043 |
| spellingShingle | Juliana Seger Link Silvana Maria Alban Carlos Ricardo Soccol Gilberto Vinicius Melo Pereira Vanete Thomaz Soccol Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis Journal of Immunology Research |
| title | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
| title_full | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
| title_fullStr | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
| title_full_unstemmed | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
| title_short | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
| title_sort | synthetic peptides as potential antigens for cutaneous leishmaniosis diagnosis |
| url | http://dx.doi.org/10.1155/2017/5871043 |
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