Fluorescence engineering in metamaterial-assisted super-resolution localization microscope
Single-molecule localization microscopies have gained much attention for their efficient realization of a sub-diffraction-limit imaging with the resolution down to the 10-nm range. In contrast to conventional localization microscopes, which rely on particular fluorescent probes in specific condition...
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-03-01
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| Series: | Nanophotonics |
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| Online Access: | https://doi.org/10.1515/nanoph-2022-0751 |
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| author | Choi Kyu Ri Li Shilong Ozerov Igor Bedu Frédéric Park Dong Hee Joo Bin Chan Wu Jeong Weon Nic Chormaic Síle Lee Yeon Ui |
| author_facet | Choi Kyu Ri Li Shilong Ozerov Igor Bedu Frédéric Park Dong Hee Joo Bin Chan Wu Jeong Weon Nic Chormaic Síle Lee Yeon Ui |
| author_sort | Choi Kyu Ri |
| collection | DOAJ |
| description | Single-molecule localization microscopies have gained much attention for their efficient realization of a sub-diffraction-limit imaging with the resolution down to the 10-nm range. In contrast to conventional localization microscopes, which rely on particular fluorescent probes in specific conditions, metamaterial-assisted super-resolution microscopies can be implemented with any fluorescent dye under general conditions. Here, we present a systematic study of fluorescence engineering in metamaterial assisted localization microscopy by using cyclic group metasurfaces coated with a fluorescent film. Tailored variations are clearly demonstrated in both the photoluminescence intensity and the photobleaching lifetime of fluorophores based on the spatially varied Purcell effect near the metasurfaces. The enhanced emissions and blinking dynamics of the fluorophores on these metasurfaces lead to an increased signal-to-noise ratio, and therefore give rise to a super-resolution localization image with 0.9-nm localization accuracy. Our results are not only beneficial for super-resolution localization imaging but also push the control of light–matter interactions beyond the diffraction limit. |
| format | Article |
| id | doaj-art-59d70b38d4d54607b526fa6b9db9e8c4 |
| institution | Kabale University |
| issn | 2192-8614 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj-art-59d70b38d4d54607b526fa6b9db9e8c42025-02-02T15:46:12ZengDe GruyterNanophotonics2192-86142023-03-0112132491249810.1515/nanoph-2022-0751Fluorescence engineering in metamaterial-assisted super-resolution localization microscopeChoi Kyu Ri0Li Shilong1Ozerov Igor2Bedu Frédéric3Park Dong Hee4Joo Bin Chan5Wu Jeong Weon6Nic Chormaic Síle7Lee Yeon Ui8Department of Physics, Chungbuk National University, Cheongju, Chungbuk28644, South KoreaLight-Matter Interactions for Quantum Technologies Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa904-0495, JapanAix-Marseille University, CNRS, CINaM UMR 7325, AMUTech, Marseille, FranceAix-Marseille University, CNRS, CINaM UMR 7325, AMUTech, Marseille, FranceDepartment of Physics, Chungbuk National University, Cheongju, Chungbuk28644, South KoreaDepartment of Physics, Chungbuk National University, Cheongju, Chungbuk28644, South KoreaDepartment of Physics, Ewha Womans University, Seoul03760, South KoreaLight-Matter Interactions for Quantum Technologies Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa904-0495, JapanDepartment of Physics, Chungbuk National University, Cheongju, Chungbuk28644, South KoreaSingle-molecule localization microscopies have gained much attention for their efficient realization of a sub-diffraction-limit imaging with the resolution down to the 10-nm range. In contrast to conventional localization microscopes, which rely on particular fluorescent probes in specific conditions, metamaterial-assisted super-resolution microscopies can be implemented with any fluorescent dye under general conditions. Here, we present a systematic study of fluorescence engineering in metamaterial assisted localization microscopy by using cyclic group metasurfaces coated with a fluorescent film. Tailored variations are clearly demonstrated in both the photoluminescence intensity and the photobleaching lifetime of fluorophores based on the spatially varied Purcell effect near the metasurfaces. The enhanced emissions and blinking dynamics of the fluorophores on these metasurfaces lead to an increased signal-to-noise ratio, and therefore give rise to a super-resolution localization image with 0.9-nm localization accuracy. Our results are not only beneficial for super-resolution localization imaging but also push the control of light–matter interactions beyond the diffraction limit.https://doi.org/10.1515/nanoph-2022-0751enhanced fluorescencemetamaterialspurcell effectsuper-resolution imaging |
| spellingShingle | Choi Kyu Ri Li Shilong Ozerov Igor Bedu Frédéric Park Dong Hee Joo Bin Chan Wu Jeong Weon Nic Chormaic Síle Lee Yeon Ui Fluorescence engineering in metamaterial-assisted super-resolution localization microscope Nanophotonics enhanced fluorescence metamaterials purcell effect super-resolution imaging |
| title | Fluorescence engineering in metamaterial-assisted super-resolution localization microscope |
| title_full | Fluorescence engineering in metamaterial-assisted super-resolution localization microscope |
| title_fullStr | Fluorescence engineering in metamaterial-assisted super-resolution localization microscope |
| title_full_unstemmed | Fluorescence engineering in metamaterial-assisted super-resolution localization microscope |
| title_short | Fluorescence engineering in metamaterial-assisted super-resolution localization microscope |
| title_sort | fluorescence engineering in metamaterial assisted super resolution localization microscope |
| topic | enhanced fluorescence metamaterials purcell effect super-resolution imaging |
| url | https://doi.org/10.1515/nanoph-2022-0751 |
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