Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy
To reveal the expression and possible role of tribbles homolog 3 (TRB3) in the incidence of type 2 diabetic nephropathy, we used immunohistochemistry, real-time quantitative PCR, western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to study the expression of TRB3, extracellular signa...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/240396 |
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| author | Luwei Zhang Jinhang Zhang Xinnong Liu Shengli Liu Jun Tian |
| author_facet | Luwei Zhang Jinhang Zhang Xinnong Liu Shengli Liu Jun Tian |
| author_sort | Luwei Zhang |
| collection | DOAJ |
| description | To reveal the expression and possible role of tribbles homolog 3 (TRB3) in the incidence of type 2 diabetic nephropathy, we used immunohistochemistry, real-time quantitative PCR, western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to study the expression of TRB3, extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase (ERK1/2 MAPK), transforming growth factor β1 (TGF-β1), and collagen type IV in kidneys of db/db diabetic mice and in murine renal mesangial cells stimulated with high glucose. The expression of TRB3, TGF-β1, and collagen type IV was increased in kidneys of db/db diabetic mice. TGF-β1 and collagen type IV regulated by high glucose through ERK1/2 MAPK were downregulated by silencing TRB3 in renal mesangial cells. TRB3 may be involved in diabetic nephropathy by regulating the fibrosis cytokine TGF-β1 and collagen type IV through the ERK1/2 MAPK signaling pathway. |
| format | Article |
| id | doaj-art-57ec592af63b401f895c2d72a50bfa2c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-57ec592af63b401f895c2d72a50bfa2c2025-02-03T06:08:35ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/240396240396Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic NephropathyLuwei Zhang0Jinhang Zhang1Xinnong Liu2Shengli Liu3Jun Tian4Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, ChinaGeneral Department, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, ChinaTo reveal the expression and possible role of tribbles homolog 3 (TRB3) in the incidence of type 2 diabetic nephropathy, we used immunohistochemistry, real-time quantitative PCR, western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to study the expression of TRB3, extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase (ERK1/2 MAPK), transforming growth factor β1 (TGF-β1), and collagen type IV in kidneys of db/db diabetic mice and in murine renal mesangial cells stimulated with high glucose. The expression of TRB3, TGF-β1, and collagen type IV was increased in kidneys of db/db diabetic mice. TGF-β1 and collagen type IV regulated by high glucose through ERK1/2 MAPK were downregulated by silencing TRB3 in renal mesangial cells. TRB3 may be involved in diabetic nephropathy by regulating the fibrosis cytokine TGF-β1 and collagen type IV through the ERK1/2 MAPK signaling pathway.http://dx.doi.org/10.1155/2014/240396 |
| spellingShingle | Luwei Zhang Jinhang Zhang Xinnong Liu Shengli Liu Jun Tian Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy Journal of Immunology Research |
| title | Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy |
| title_full | Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy |
| title_fullStr | Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy |
| title_full_unstemmed | Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy |
| title_short | Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy |
| title_sort | tribbles 3 regulates the fibrosis cytokine tgf β1 through erk1 2 mapk signaling pathway in diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2014/240396 |
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