The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation
Purpose. CD4+LAP+ T cells are newly discovered regulatory T cells (Tregs). The aim of this study is to investigate the balance of Th1/Th2 and LAP+Tregs/Th17 in mice after allogeneic corneal transplantation. Methods. A total of 65 mice received orthotopic penetrating transplantation. According to the...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/5404989 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552431440887808 |
|---|---|
| author | Shang Li Jing Yu Chungang Guo Ying Jie Zhiqiang Pan |
| author_facet | Shang Li Jing Yu Chungang Guo Ying Jie Zhiqiang Pan |
| author_sort | Shang Li |
| collection | DOAJ |
| description | Purpose. CD4+LAP+ T cells are newly discovered regulatory T cells (Tregs). The aim of this study is to investigate the balance of Th1/Th2 and LAP+Tregs/Th17 in mice after allogeneic corneal transplantation. Methods. A total of 65 mice received orthotopic penetrating transplantation. According to the survival scores of the grafts, the mice were divided into the rejection group and the survival group 3 weeks after transplantation. Th1, Th2, Th17, and regulatory T cells in the ipsilateral drainage lymph nodes and spleens were measured with flow cytometry. The related cytokines in aqueous humor were also analyzed. Results. The frequencies of Foxp3+Tregs, GARP+Tregs, and LAP+Tregs in the survival group were significantly higher than those in the rejection group. And the expression trend of CD4+LAP+ T cells and CD4+GARP+ T cells was consistent. The level of IFN-γ, TNF, IL-6, and IL-17A markedly increased in aqueous humor during corneal allograft rejection. The ratio of Th1/Th2 and Th17/LAP+Tregs significantly increased in the rejection group at the 3rd week after corneal transplantation. Conclusion. LAP+Tregs might be regarded as substitute for Foxp3+Tregs. The balance of Th1/Th2 and LAP+Tregs/Th17 is crucial for corneal allograft survival. |
| format | Article |
| id | doaj-art-56c9810237a54e2e8269a24a7ae896b5 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-56c9810237a54e2e8269a24a7ae896b52025-02-03T05:58:39ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/54049895404989The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal TransplantationShang Li0Jing Yu1Chungang Guo2Ying Jie3Zhiqiang Pan4Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Ophthalmology, Beijing YouAn Hospital, Capital Medical University, Beijing, ChinaDepartment of Ophthalmology, Beijing YouAn Hospital, Capital Medical University, Beijing, ChinaBeijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaBeijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaPurpose. CD4+LAP+ T cells are newly discovered regulatory T cells (Tregs). The aim of this study is to investigate the balance of Th1/Th2 and LAP+Tregs/Th17 in mice after allogeneic corneal transplantation. Methods. A total of 65 mice received orthotopic penetrating transplantation. According to the survival scores of the grafts, the mice were divided into the rejection group and the survival group 3 weeks after transplantation. Th1, Th2, Th17, and regulatory T cells in the ipsilateral drainage lymph nodes and spleens were measured with flow cytometry. The related cytokines in aqueous humor were also analyzed. Results. The frequencies of Foxp3+Tregs, GARP+Tregs, and LAP+Tregs in the survival group were significantly higher than those in the rejection group. And the expression trend of CD4+LAP+ T cells and CD4+GARP+ T cells was consistent. The level of IFN-γ, TNF, IL-6, and IL-17A markedly increased in aqueous humor during corneal allograft rejection. The ratio of Th1/Th2 and Th17/LAP+Tregs significantly increased in the rejection group at the 3rd week after corneal transplantation. Conclusion. LAP+Tregs might be regarded as substitute for Foxp3+Tregs. The balance of Th1/Th2 and LAP+Tregs/Th17 is crucial for corneal allograft survival.http://dx.doi.org/10.1155/2018/5404989 |
| spellingShingle | Shang Li Jing Yu Chungang Guo Ying Jie Zhiqiang Pan The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation Journal of Ophthalmology |
| title | The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation |
| title_full | The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation |
| title_fullStr | The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation |
| title_full_unstemmed | The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation |
| title_short | The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation |
| title_sort | balance of th1 th2 and lap tregs th17 cells is crucial for graft survival in allogeneic corneal transplantation |
| url | http://dx.doi.org/10.1155/2018/5404989 |
| work_keys_str_mv | AT shangli thebalanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT jingyu thebalanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT chungangguo thebalanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT yingjie thebalanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT zhiqiangpan thebalanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT shangli balanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT jingyu balanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT chungangguo balanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT yingjie balanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation AT zhiqiangpan balanceofth1th2andlaptregsth17cellsiscrucialforgraftsurvivalinallogeneiccornealtransplantation |