DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye
Mosquitoes play a critical role as disease vectors, making them significant in terms of both public health concerns and ecological balance. This study aims to identify mosquito specimens collected from Siirt city center and six different districts using morphological and molecular methods. A 658 bp...
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| Format: | Article |
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Turkish Science and Technology Publishing (TURSTEP)
2025-01-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/7197 |
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| author | Halil Dilmen Behcet İnal Mehmet Salih Özgökçe Mustafa Cemal Çiftçi Hilmi Kara Sedriye Çatkın Meryem Özer Dilmen Gülcihan Koyunçu |
| author_facet | Halil Dilmen Behcet İnal Mehmet Salih Özgökçe Mustafa Cemal Çiftçi Hilmi Kara Sedriye Çatkın Meryem Özer Dilmen Gülcihan Koyunçu |
| author_sort | Halil Dilmen |
| collection | DOAJ |
| description | Mosquitoes play a critical role as disease vectors, making them significant in terms of both public health concerns and ecological balance. This study aims to identify mosquito specimens collected from Siirt city center and six different districts using morphological and molecular methods. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region was used for molecular diagnosis. The findings revealed four mosquito species: Culex theileri Theobald, 1903, Culex mimeticus Noè, 1899, Culex quinquefasciatus Say, 1823, and Anopheles superpictus Grassi, 1899. Mitochondrial gene PCR products were sequenced, and the sequences were uploaded to the NCBI database for public access. Phylogenetic analysis was conducted using these sequences to investigate the genetic distances and evolutionary relationships among the mosquito species. In the phylogenetic analysis, Chironomus kiiensis was used as an outgroup. The analysis revealed that C. quinquefasciatus and A. superpictus were had the highest genetic distance (0.16), while the closest genetic distance was observed between C. quinquefasciatus and C. theileri (0.06). This study is presented as a preliminary investigation into the genetic diversity, evolutionary relationships, and population dynamics of mosquito species in Siirt Province. Further studies with a larger sample size and additional sequences are needed to establish more comprehensive phylogenetic relationships. The molecular findings contribute significantly to the systematic and ecological studies of mosquitoes. |
| format | Article |
| id | doaj-art-5653e583bd1a4514b1b17e073e6d016b |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-5653e583bd1a4514b1b17e073e6d016b2025-02-06T13:44:57ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2025-01-0113133425898DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, TürkiyeHalil Dilmen0https://orcid.org/0000-0002-3802-9947Behcet İnal1https://orcid.org/0000-0003-2215-2710Mehmet Salih Özgökçe2https://orcid.org/0000-0002-6777-9149Mustafa Cemal Çiftçi3https://orcid.org/0000-0003-2386-6969Hilmi Kara4https://orcid.org/0000-0003-0580-0464Sedriye Çatkın5https://orcid.org/0000-0002-5384-1079Meryem Özer Dilmen6https://orcid.org/0000-0003-0202-6362Gülcihan Koyunçu7https://orcid.org/0009-0000-4789-4644Siirt University, Faculty of Agricultura, Department of Plant Protection, 56100, Siirt, TürkiyeSiirt University, Faculty of Agricultura, Department of Agricultural BiotechnologyVan Yüzüncü Yıl University, Faculty of Agricultura, Department of Plant ProtectionSiirt University, Faculty of Agricultura, Department of Plant ProtectionVan Yüzüncü Yıl University, Faculty of Agricultura, Department of Plant ProtectionSiirt University, Faculty of Agricultura, Department of Agricultural BiotechnologySiirt University, Kurtalan Vocational High School, Department of Plant and Animal Production Van Yüzüncü Yıl University, Faculty of Agricultura, Department of Plant ProtectionMosquitoes play a critical role as disease vectors, making them significant in terms of both public health concerns and ecological balance. This study aims to identify mosquito specimens collected from Siirt city center and six different districts using morphological and molecular methods. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region was used for molecular diagnosis. The findings revealed four mosquito species: Culex theileri Theobald, 1903, Culex mimeticus Noè, 1899, Culex quinquefasciatus Say, 1823, and Anopheles superpictus Grassi, 1899. Mitochondrial gene PCR products were sequenced, and the sequences were uploaded to the NCBI database for public access. Phylogenetic analysis was conducted using these sequences to investigate the genetic distances and evolutionary relationships among the mosquito species. In the phylogenetic analysis, Chironomus kiiensis was used as an outgroup. The analysis revealed that C. quinquefasciatus and A. superpictus were had the highest genetic distance (0.16), while the closest genetic distance was observed between C. quinquefasciatus and C. theileri (0.06). This study is presented as a preliminary investigation into the genetic diversity, evolutionary relationships, and population dynamics of mosquito species in Siirt Province. Further studies with a larger sample size and additional sequences are needed to establish more comprehensive phylogenetic relationships. The molecular findings contribute significantly to the systematic and ecological studies of mosquitoes.https://agrifoodscience.com/index.php/TURJAF/article/view/7197coi geneculicidaemolecular identificationdna barcodingspecies identification |
| spellingShingle | Halil Dilmen Behcet İnal Mehmet Salih Özgökçe Mustafa Cemal Çiftçi Hilmi Kara Sedriye Çatkın Meryem Özer Dilmen Gülcihan Koyunçu DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye Turkish Journal of Agriculture: Food Science and Technology coi gene culicidae molecular identification dna barcoding species identification |
| title | DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye |
| title_full | DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye |
| title_fullStr | DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye |
| title_full_unstemmed | DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye |
| title_short | DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye |
| title_sort | dna barcoding and phylogenetic analysis of culex and anopheles species in siirt turkiye |
| topic | coi gene culicidae molecular identification dna barcoding species identification |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/7197 |
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