Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC
A pre-column derivatization high-performance liquid chromatography (HPLC) method was developed and optimized to characterize and quantify the monosaccharides present in tea polysaccharides (TPS) isolated from Yingshuang green tea. TPS sample was hydrolyzed with trifluoroacetic acid, subjected to pre...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/6065813 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548117387411456 |
|---|---|
| author | Yujie Ai Zhi Yu Yuqiong Chen Xiaojing Zhu Zeyi Ai Shuyuan Liu Dejiang Ni |
| author_facet | Yujie Ai Zhi Yu Yuqiong Chen Xiaojing Zhu Zeyi Ai Shuyuan Liu Dejiang Ni |
| author_sort | Yujie Ai |
| collection | DOAJ |
| description | A pre-column derivatization high-performance liquid chromatography (HPLC) method was developed and optimized to characterize and quantify the monosaccharides present in tea polysaccharides (TPS) isolated from Yingshuang green tea. TPS sample was hydrolyzed with trifluoroacetic acid, subjected to pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone (PMP), and separated on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with UV detection at 250 nm. A mixture of ten PMP derivatives of standard monosaccharides (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, arabinose, and fucose) could be baseline separated within 20 min. Moreover, quantitative analysis of the component monosaccharides in Yingshuang green tea TPS was achieved, indicating the TPS consisted of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, and arabinose in the molar contents of 0.72, 0.78, 0.89, 0.13, 0.15, 0.36, 0.39, 0.36, 0.36, and 0.38 μM, respectively. Recovery efficiency for component monosaccharides from TPS ranged from 93.6 to 102.4% with RSD values lower than 2.5%. In conclusion, pre-column derivatization HPLC provides a rapid, reproducible, accurate, and quantitative method for analysis of the monosaccharide composition and contents in TPS, which may help to further explore the relationship between TPS monosaccharides isolated from different tea varieties and their biological activity. |
| format | Article |
| id | doaj-art-55c29977c76d40fba4df1d209d05c698 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-55c29977c76d40fba4df1d209d05c6982025-02-03T06:42:11ZengWileyJournal of Chemistry2090-90632090-90712016-01-01201610.1155/2016/60658136065813Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLCYujie Ai0Zhi Yu1Yuqiong Chen2Xiaojing Zhu3Zeyi Ai4Shuyuan Liu5Dejiang Ni6Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaA pre-column derivatization high-performance liquid chromatography (HPLC) method was developed and optimized to characterize and quantify the monosaccharides present in tea polysaccharides (TPS) isolated from Yingshuang green tea. TPS sample was hydrolyzed with trifluoroacetic acid, subjected to pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone (PMP), and separated on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with UV detection at 250 nm. A mixture of ten PMP derivatives of standard monosaccharides (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, arabinose, and fucose) could be baseline separated within 20 min. Moreover, quantitative analysis of the component monosaccharides in Yingshuang green tea TPS was achieved, indicating the TPS consisted of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, and arabinose in the molar contents of 0.72, 0.78, 0.89, 0.13, 0.15, 0.36, 0.39, 0.36, 0.36, and 0.38 μM, respectively. Recovery efficiency for component monosaccharides from TPS ranged from 93.6 to 102.4% with RSD values lower than 2.5%. In conclusion, pre-column derivatization HPLC provides a rapid, reproducible, accurate, and quantitative method for analysis of the monosaccharide composition and contents in TPS, which may help to further explore the relationship between TPS monosaccharides isolated from different tea varieties and their biological activity.http://dx.doi.org/10.1155/2016/6065813 |
| spellingShingle | Yujie Ai Zhi Yu Yuqiong Chen Xiaojing Zhu Zeyi Ai Shuyuan Liu Dejiang Ni Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC Journal of Chemistry |
| title | Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC |
| title_full | Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC |
| title_fullStr | Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC |
| title_full_unstemmed | Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC |
| title_short | Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC |
| title_sort | rapid determination of the monosaccharide composition and contents in tea polysaccharides from yingshuang green tea by pre column derivatization hplc |
| url | http://dx.doi.org/10.1155/2016/6065813 |
| work_keys_str_mv | AT yujieai rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT zhiyu rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT yuqiongchen rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT xiaojingzhu rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT zeyiai rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT shuyuanliu rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc AT dejiangni rapiddeterminationofthemonosaccharidecompositionandcontentsinteapolysaccharidesfromyingshuanggreenteabyprecolumnderivatizationhplc |