Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm
Abstract This study assessed the effect of composite resins, aggregated or not with S-PRG particles, and the use of toothpaste in controlling demineralization and bacterial growth. Human molars were distributed into 3 groups: control (CT) – sound teeth, Beautifil Bulk Restorative System (aggregated...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-78396-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594828415729664 |
|---|---|
| author | Andrea Freire Victor Augusto Alves Bento Eduardo Inocente Jussiani Avacir Casanova Andrello Maria Carolina Silva Marques |
| author_facet | Andrea Freire Victor Augusto Alves Bento Eduardo Inocente Jussiani Avacir Casanova Andrello Maria Carolina Silva Marques |
| author_sort | Andrea Freire |
| collection | DOAJ |
| description | Abstract This study assessed the effect of composite resins, aggregated or not with S-PRG particles, and the use of toothpaste in controlling demineralization and bacterial growth. Human molars were distributed into 3 groups: control (CT) – sound teeth, Beautifil Bulk Restorative System (aggregated with S-PRG) (BB), Filtek One Bulk Fill (without S-PRG) (FB). Teeth destined for groups BB and FB previously received Class I preparations (4 × 4 × 4 mm), followed by single-increment restorations. All teeth were sectioned mesiodistally, with all specimens subjected to cariogenic challenge for 5 days, including microcosm biofilm formation. Half of each tooth was exposed to toothpaste (CTF, BBF, FBF). The loss of microhardness was assessed considering the initial microhardness as 100% on enamel, dentin, and composite resin substrates. Colony Forming Units (CFU/mL) were counted in 3 media. Data analysis used one-way ANOVA, Tukey HSD test, and paired t-test (α = 0.05). Toothpaste significantly reduced CFU/mL for total bacteria and genus Streptococcus (p < 0.05), with no significant difference for Streptococcus mutans. Enamel microhardness was positively affected by toothpaste. Both restorative systems controlled enamel demineralization, with FB and FBF outperforming BB and BBF. There was minor degradation of both composite resins, between 10% and 22%. Toothpaste effectively reduced microorganisms, irrespective of the composite resin. Regarding demineralization control, both restorative systems, with and without S-PRG particles, were effective on enamel. |
| format | Article |
| id | doaj-art-557b99fb1f49488fb6cdc2c4a1743eb8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-557b99fb1f49488fb6cdc2c4a1743eb82025-01-19T12:17:04ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-78396-1Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilmAndrea Freire0Victor Augusto Alves Bento1Eduardo Inocente Jussiani2Avacir Casanova Andrello3Maria Carolina Silva Marques4School of Dentistry, Universidade Federal de Mato Grosso do SulSchool of Dentistry, Universidade Federal de Mato Grosso do SulApplied Nuclear Physics Research Group, State Univerisity of LondrinaApplied Nuclear Physics Research Group, State Univerisity of LondrinaInstitute of Biology (Inbio), Universidade Federal de Mato Grosso do SulAbstract This study assessed the effect of composite resins, aggregated or not with S-PRG particles, and the use of toothpaste in controlling demineralization and bacterial growth. Human molars were distributed into 3 groups: control (CT) – sound teeth, Beautifil Bulk Restorative System (aggregated with S-PRG) (BB), Filtek One Bulk Fill (without S-PRG) (FB). Teeth destined for groups BB and FB previously received Class I preparations (4 × 4 × 4 mm), followed by single-increment restorations. All teeth were sectioned mesiodistally, with all specimens subjected to cariogenic challenge for 5 days, including microcosm biofilm formation. Half of each tooth was exposed to toothpaste (CTF, BBF, FBF). The loss of microhardness was assessed considering the initial microhardness as 100% on enamel, dentin, and composite resin substrates. Colony Forming Units (CFU/mL) were counted in 3 media. Data analysis used one-way ANOVA, Tukey HSD test, and paired t-test (α = 0.05). Toothpaste significantly reduced CFU/mL for total bacteria and genus Streptococcus (p < 0.05), with no significant difference for Streptococcus mutans. Enamel microhardness was positively affected by toothpaste. Both restorative systems controlled enamel demineralization, with FB and FBF outperforming BB and BBF. There was minor degradation of both composite resins, between 10% and 22%. Toothpaste effectively reduced microorganisms, irrespective of the composite resin. Regarding demineralization control, both restorative systems, with and without S-PRG particles, were effective on enamel.https://doi.org/10.1038/s41598-024-78396-1DemineralizationResin compositeToothpasteDental cariesSecondary caries |
| spellingShingle | Andrea Freire Victor Augusto Alves Bento Eduardo Inocente Jussiani Avacir Casanova Andrello Maria Carolina Silva Marques Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm Scientific Reports Demineralization Resin composite Toothpaste Dental caries Secondary caries |
| title | Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm |
| title_full | Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm |
| title_fullStr | Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm |
| title_full_unstemmed | Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm |
| title_short | Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm |
| title_sort | resin composite aggregated s prg particles are not superior to non s prg under microcosm biofilm |
| topic | Demineralization Resin composite Toothpaste Dental caries Secondary caries |
| url | https://doi.org/10.1038/s41598-024-78396-1 |
| work_keys_str_mv | AT andreafreire resincompositeaggregatedsprgparticlesarenotsuperiortononsprgundermicrocosmbiofilm AT victoraugustoalvesbento resincompositeaggregatedsprgparticlesarenotsuperiortononsprgundermicrocosmbiofilm AT eduardoinocentejussiani resincompositeaggregatedsprgparticlesarenotsuperiortononsprgundermicrocosmbiofilm AT avacircasanovaandrello resincompositeaggregatedsprgparticlesarenotsuperiortononsprgundermicrocosmbiofilm AT mariacarolinasilvamarques resincompositeaggregatedsprgparticlesarenotsuperiortononsprgundermicrocosmbiofilm |