An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase
Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic food. In this work, a polydopamine (PDA)-based fluorescent aptasensor was developed to detect arginine kinase (AK), one of the major allergens in shellfish. The aptamer t...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302200194X |
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| author | Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu |
| author_facet | Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu |
| author_sort | Yanbo Wang |
| collection | DOAJ |
| description | Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic food. In this work, a polydopamine (PDA)-based fluorescent aptasensor was developed to detect arginine kinase (AK), one of the major allergens in shellfish. The aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite (FAM) to build a fluorescence resonance energy transfer (FRET) system with PDA particles. Polyethylene glycol (PEG) was employed to construct an antifouling surface for the aptasensor to eliminate food matrix interferences. With the presence of AK, the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA particles. The aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection (LOD) of 0.298 μg/mL. The proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products, which indicated its high potential in tracing the presence of AK in complex aquatic products. |
| format | Article |
| id | doaj-art-555ba969e9dd40cd976f6af1aa89d3e0 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-555ba969e9dd40cd976f6af1aa89d3e02025-02-03T10:34:09ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123737744An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinaseYanbo Wang0Huan Li1Jinru Zhou2Fangting Wang3Yifan Qian4Linglin Fu5Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaFood Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaCorresponding author at: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xue Zheng Street, Hangzhou 310018, China.; Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaSimple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic food. In this work, a polydopamine (PDA)-based fluorescent aptasensor was developed to detect arginine kinase (AK), one of the major allergens in shellfish. The aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite (FAM) to build a fluorescence resonance energy transfer (FRET) system with PDA particles. Polyethylene glycol (PEG) was employed to construct an antifouling surface for the aptasensor to eliminate food matrix interferences. With the presence of AK, the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA particles. The aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection (LOD) of 0.298 μg/mL. The proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products, which indicated its high potential in tracing the presence of AK in complex aquatic products.http://www.sciencedirect.com/science/article/pii/S221345302200194XFood allergyAptasensorAntifoulingPolydopamineFRET effect |
| spellingShingle | Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase Food Science and Human Wellness Food allergy Aptasensor Antifouling Polydopamine FRET effect |
| title | An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase |
| title_full | An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase |
| title_fullStr | An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase |
| title_full_unstemmed | An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase |
| title_short | An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase |
| title_sort | antifouling polydopamine based fluorescent aptasensor for determination of arginine kinase |
| topic | Food allergy Aptasensor Antifouling Polydopamine FRET effect |
| url | http://www.sciencedirect.com/science/article/pii/S221345302200194X |
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