A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues
The existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures. Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-pen...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2017/9043134 |
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| _version_ | 1832560807612776448 |
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| author | Xiangpei Yue Yaoyue Zhang Wen Xing Yutong Chen Chenyang Mu Zhan Miao Peichun Ge Tingting Li Rongqiao He Zhiqian Tong |
| author_facet | Xiangpei Yue Yaoyue Zhang Wen Xing Yutong Chen Chenyang Mu Zhan Miao Peichun Ge Tingting Li Rongqiao He Zhiqian Tong |
| author_sort | Xiangpei Yue |
| collection | DOAJ |
| description | The existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures. Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4-dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature. The sensitive response time point was found to be at the first hour, and the optimal pH of derivative reaction was pH 6.0. The limit of detection (LOD) and the limits of quantization (LOQ) for detecting FA were 0.5 μM and 2.5 μM, respectively. Using this method, an abnormally high level of FA was detected in both the brains of FA-injected mice and autopsy hippocampus tissues from patients with Alzheimer’s disease. This finding suggests that the modified Fluoral-P method is effective for measuring levels of FA in the brains. |
| format | Article |
| id | doaj-art-54db902054b24af9b28f0b93caeb3b86 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-54db902054b24af9b28f0b93caeb3b862025-02-03T01:26:39ZengWileyAnalytical Cellular Pathology2210-71772210-71852017-01-01201710.1155/2017/90431349043134A Sensitive and Rapid Method for Detecting Formaldehyde in Brain TissuesXiangpei Yue0Yaoyue Zhang1Wen Xing2Yutong Chen3Chenyang Mu4Zhan Miao5Peichun Ge6Tingting Li7Rongqiao He8Zhiqian Tong9Alzheimer’s Disease Center, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaBeijing No. 12 Laboratory of Brain and Cognitive Sciences, Beijing 100071, ChinaAlzheimer’s Disease Center, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, ChinaAlzheimer’s Disease Center, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, ChinaThe existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures. Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4-dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature. The sensitive response time point was found to be at the first hour, and the optimal pH of derivative reaction was pH 6.0. The limit of detection (LOD) and the limits of quantization (LOQ) for detecting FA were 0.5 μM and 2.5 μM, respectively. Using this method, an abnormally high level of FA was detected in both the brains of FA-injected mice and autopsy hippocampus tissues from patients with Alzheimer’s disease. This finding suggests that the modified Fluoral-P method is effective for measuring levels of FA in the brains.http://dx.doi.org/10.1155/2017/9043134 |
| spellingShingle | Xiangpei Yue Yaoyue Zhang Wen Xing Yutong Chen Chenyang Mu Zhan Miao Peichun Ge Tingting Li Rongqiao He Zhiqian Tong A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues Analytical Cellular Pathology |
| title | A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues |
| title_full | A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues |
| title_fullStr | A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues |
| title_full_unstemmed | A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues |
| title_short | A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues |
| title_sort | sensitive and rapid method for detecting formaldehyde in brain tissues |
| url | http://dx.doi.org/10.1155/2017/9043134 |
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