Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence
Abstract Extracellular vesicles (EVs) are membrane‐bound exosomes secreted into the apoplast. Two distinct populations of EVs have been described in Arabidopsis: PEN1‐associated and TET8‐associated. We previously noted early leaf senescence in the pen1 single and pen1pen3 double mutant. Both PEN1 an...
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| Format: | Article |
| Language: | English |
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Wiley
2024-09-01
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| Series: | Plant Direct |
| Online Access: | https://doi.org/10.1002/pld3.70006 |
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| author | Jayde A. Zimmerman Benjamin Verboonen Andrew P. Harrison Hanson Luis R. Arballo Judy A. Brusslan |
| author_facet | Jayde A. Zimmerman Benjamin Verboonen Andrew P. Harrison Hanson Luis R. Arballo Judy A. Brusslan |
| author_sort | Jayde A. Zimmerman |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are membrane‐bound exosomes secreted into the apoplast. Two distinct populations of EVs have been described in Arabidopsis: PEN1‐associated and TET8‐associated. We previously noted early leaf senescence in the pen1 single and pen1pen3 double mutant. Both PEN1 and PEN3 are abundant in EV proteomes suggesting that EVs might regulate leaf senescence in soil‐grown plants. We observed that TET8 is more abundant in the apoplast of early senescing pen1 and pen1pen3 mutant rosettes and in older wild‐type (WT) rosettes. The increase in apoplast TET8 in the pen1 mutant did not correspond to increased TET8 mRNA levels. In addition, apoplast TET8 was more abundant in the early leaf senescence myb59 mutant, meaning the increase in apoplast TET8 protein during leaf senescence is not dependent on pen1 or pen3. Genetic analysis showed a significant delay in leaf senescence in tet3tet8 double mutants after 6 weeks of growth suggesting that these two tetraspanin paralogs operate additively and are positive regulators of leaf senescence. This is opposite of the effect of pen1 and pen1pen3 mutants that show early senescence and suggest PEN1 to be a negative regulator of leaf senescence. Our work provides initial support that apoplast‐localized TET8 in combination with TET3 positively regulates age‐related leaf senescence in soil‐grown Arabidopsis plants. |
| format | Article |
| id | doaj-art-53a4861c91c04308a15433b18eeb1b2c |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-53a4861c91c04308a15433b18eeb1b2c2025-01-20T03:56:13ZengWileyPlant Direct2475-44552024-09-0189n/an/a10.1002/pld3.70006Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescenceJayde A. Zimmerman0Benjamin Verboonen1Andrew P. Harrison Hanson2Luis R. Arballo3Judy A. Brusslan4Southern California Coastal Water Research Project Costa Mesa California USAUniversity of California, Riverside Riverside California USABurrell College of Osteopathic Medicine Melbourne Florida USACalifornia State University, Long Beach Long Beach California USADepartment of Biological Sciences California State University, Long Beach Long Beach California USAAbstract Extracellular vesicles (EVs) are membrane‐bound exosomes secreted into the apoplast. Two distinct populations of EVs have been described in Arabidopsis: PEN1‐associated and TET8‐associated. We previously noted early leaf senescence in the pen1 single and pen1pen3 double mutant. Both PEN1 and PEN3 are abundant in EV proteomes suggesting that EVs might regulate leaf senescence in soil‐grown plants. We observed that TET8 is more abundant in the apoplast of early senescing pen1 and pen1pen3 mutant rosettes and in older wild‐type (WT) rosettes. The increase in apoplast TET8 in the pen1 mutant did not correspond to increased TET8 mRNA levels. In addition, apoplast TET8 was more abundant in the early leaf senescence myb59 mutant, meaning the increase in apoplast TET8 protein during leaf senescence is not dependent on pen1 or pen3. Genetic analysis showed a significant delay in leaf senescence in tet3tet8 double mutants after 6 weeks of growth suggesting that these two tetraspanin paralogs operate additively and are positive regulators of leaf senescence. This is opposite of the effect of pen1 and pen1pen3 mutants that show early senescence and suggest PEN1 to be a negative regulator of leaf senescence. Our work provides initial support that apoplast‐localized TET8 in combination with TET3 positively regulates age‐related leaf senescence in soil‐grown Arabidopsis plants.https://doi.org/10.1002/pld3.70006 |
| spellingShingle | Jayde A. Zimmerman Benjamin Verboonen Andrew P. Harrison Hanson Luis R. Arballo Judy A. Brusslan Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence Plant Direct |
| title | Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence |
| title_full | Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence |
| title_fullStr | Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence |
| title_full_unstemmed | Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence |
| title_short | Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence |
| title_sort | arabidopsis apoplast tet8 positively correlates to leaf senescence and tet3tet8 double mutants are delayed in leaf senescence |
| url | https://doi.org/10.1002/pld3.70006 |
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