Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy
In plants, the first interaction between the pollen grain and the epidermal cells of the stigma is crucial for successful reproduction. When the pollen is accepted, it germinates, producing a tube that transports the two sperm cells to the ovules for fertilization. Confocal microscopy has been used...
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Bio-protocol LLC
2024-11-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=5110&type=0 |
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| author | Lucie Riglet Isabelle Fobis-Loisy |
| author_facet | Lucie Riglet Isabelle Fobis-Loisy |
| author_sort | Lucie Riglet |
| collection | DOAJ |
| description | In plants, the first interaction between the pollen grain and the epidermal cells of the stigma is crucial for successful reproduction. When the pollen is accepted, it germinates, producing a tube that transports the two sperm cells to the ovules for fertilization. Confocal microscopy has been used to characterize the behavior of stigmatic cells post-pollination [1], but it is time-consuming since it requires the development of a range of fluorescent marker lines. Here, we propose a quick, high-resolution imaging protocol using tabletop scanning electron microscopy. This technique does not require prior sample fixation or fluorescent marker lines. It effectively captures pollen grain behavior from early hydration (a few minutes after pollination) to pollen tube growth within the stigma (1 h after pollination) and is particularly efficient for tracking pollen tube paths. |
| format | Article |
| id | doaj-art-5347b51f5a1f453ab2e9fd40b7ca29c1 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bio-protocol LLC |
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| series | Bio-Protocol |
| spelling | doaj-art-5347b51f5a1f453ab2e9fd40b7ca29c12025-08-20T02:35:30ZengBio-protocol LLCBio-Protocol2331-83252024-11-01142210.21769/BioProtoc.5110Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron MicroscopyLucie Riglet0Isabelle Fobis-Loisy1The Sainsbury Laboratory, University of Cambridge, 47 Bateman Street, Cambridge, UKLaboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE, Lyon, FranceLaboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE, Lyon, FranceIn plants, the first interaction between the pollen grain and the epidermal cells of the stigma is crucial for successful reproduction. When the pollen is accepted, it germinates, producing a tube that transports the two sperm cells to the ovules for fertilization. Confocal microscopy has been used to characterize the behavior of stigmatic cells post-pollination [1], but it is time-consuming since it requires the development of a range of fluorescent marker lines. Here, we propose a quick, high-resolution imaging protocol using tabletop scanning electron microscopy. This technique does not require prior sample fixation or fluorescent marker lines. It effectively captures pollen grain behavior from early hydration (a few minutes after pollination) to pollen tube growth within the stigma (1 h after pollination) and is particularly efficient for tracking pollen tube paths.https://bio-protocol.org/en/bpdetail?id=5110&type=0 |
| spellingShingle | Lucie Riglet Isabelle Fobis-Loisy Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy Bio-Protocol |
| title | Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy |
| title_full | Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy |
| title_fullStr | Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy |
| title_full_unstemmed | Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy |
| title_short | Fast and High-Resolution Imaging of Pollinated Stigmatic Cells by Tabletop Scanning Electron Microscopy |
| title_sort | fast and high resolution imaging of pollinated stigmatic cells by tabletop scanning electron microscopy |
| url | https://bio-protocol.org/en/bpdetail?id=5110&type=0 |
| work_keys_str_mv | AT lucieriglet fastandhighresolutionimagingofpollinatedstigmaticcellsbytabletopscanningelectronmicroscopy AT isabellefobisloisy fastandhighresolutionimagingofpollinatedstigmaticcellsbytabletopscanningelectronmicroscopy |