The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L.
Terpene trilactones (TTLs) are important secondary metabolites in ginkgo tree (Ginkgo biloba), which play a crucial role in the treatment of human cardiovascular disease and prevention of thrombosis, and have been widely used in food, medicine and health products. However, there are few studies on t...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662825000179 |
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| author | Nuo Wang Yi Tu Sirui Zeng Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao |
| author_facet | Nuo Wang Yi Tu Sirui Zeng Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao |
| author_sort | Nuo Wang |
| collection | DOAJ |
| description | Terpene trilactones (TTLs) are important secondary metabolites in ginkgo tree (Ginkgo biloba), which play a crucial role in the treatment of human cardiovascular disease and prevention of thrombosis, and have been widely used in food, medicine and health products. However, there are few studies on the transcriptional modulation of TTLs biosynthesis and gene regulatory network of TTLs biosynthesis remains unclear. Here, we screened the transcription factor GbbZIP41 that may be involved in the biosynthesis of TTLs and verified its function by transgenic technology. The results showed that GbbZIP41 is a protein located in the nucleus and encodes 477 amino acids, which is highly expressed in G. biloba leaves. The analysis of total terpene content and GbbZIP41 gene expression in G. biloba leaves showed the opposite trend. In addition, the overexpression of GbbZIP41 gene in tobacco reduced the content of terpenoids and down-regulated the enzyme genes of MVA pathway and MEP pathway. Therefore, these results suggested that GbbZIP41 negatively regulates the biosynthesis of TTLs by inhibiting key enzyme genes in the TTLs synthesis pathway. |
| format | Article |
| id | doaj-art-532d447fc21443909499468cc4e4b2ca |
| institution | Kabale University |
| issn | 2214-6628 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj-art-532d447fc21443909499468cc4e4b2ca2025-02-06T05:11:49ZengElsevierCurrent Plant Biology2214-66282025-03-0141100449The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L.Nuo Wang0Yi Tu1Sirui Zeng2Jiabao Ye3Weiwei Zhang4Feng Xu5Yongling Liao6College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCorresponding authors.; College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCorresponding authors.; College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaTerpene trilactones (TTLs) are important secondary metabolites in ginkgo tree (Ginkgo biloba), which play a crucial role in the treatment of human cardiovascular disease and prevention of thrombosis, and have been widely used in food, medicine and health products. However, there are few studies on the transcriptional modulation of TTLs biosynthesis and gene regulatory network of TTLs biosynthesis remains unclear. Here, we screened the transcription factor GbbZIP41 that may be involved in the biosynthesis of TTLs and verified its function by transgenic technology. The results showed that GbbZIP41 is a protein located in the nucleus and encodes 477 amino acids, which is highly expressed in G. biloba leaves. The analysis of total terpene content and GbbZIP41 gene expression in G. biloba leaves showed the opposite trend. In addition, the overexpression of GbbZIP41 gene in tobacco reduced the content of terpenoids and down-regulated the enzyme genes of MVA pathway and MEP pathway. Therefore, these results suggested that GbbZIP41 negatively regulates the biosynthesis of TTLs by inhibiting key enzyme genes in the TTLs synthesis pathway.http://www.sciencedirect.com/science/article/pii/S2214662825000179Ginkgo bilobaGbbZIP41Transcriptional regulationTerpene trilactones biosynthesis |
| spellingShingle | Nuo Wang Yi Tu Sirui Zeng Jiabao Ye Weiwei Zhang Feng Xu Yongling Liao The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. Current Plant Biology Ginkgo biloba GbbZIP41 Transcriptional regulation Terpene trilactones biosynthesis |
| title | The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. |
| title_full | The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. |
| title_fullStr | The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. |
| title_full_unstemmed | The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. |
| title_short | The GbbZIP41 transcription factor participates in terpene trilactones biosynthesis in Ginkgo biloba L. |
| title_sort | gbbzip41 transcription factor participates in terpene trilactones biosynthesis in ginkgo biloba l |
| topic | Ginkgo biloba GbbZIP41 Transcriptional regulation Terpene trilactones biosynthesis |
| url | http://www.sciencedirect.com/science/article/pii/S2214662825000179 |
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