The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles
The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from <i>Salmonella enterica</i> sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS,...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/10/1/55 |
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| Summary: | The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from <i>Salmonella enterica</i> sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS, in the range of 0/20/50 % <i>w</i>/<i>w</i> relative to the lipid, had a regulatory role in the structure of the LUVs toward the lower size, low polydispersity, and over-a-month size stability due to the lower negative zeta potential. The addition of LPS resulted in increased density, which determined the ultrasound velocity and the specific adiabatic compressibility. In a 0.5/1/2 mg/mL concentration range, the total lipid content did not significantly affect the size of LUVs and influenced the density-related attributes similarly to the LPS content. A positive correlation was found between temperature and vesicle size, and a negative correlation was found between temperature and density and compressibility—except for the anomaly behavior at 25 °C, around the melting point of DMPC. |
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| ISSN: | 2313-7673 |