Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts
Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefo...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/109876 |
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| author | Takuya Yamada Kentaro Uchida Kenji Onuma Jun Kuzuno Masanobu Ujihira Gen Inoue Bunpei Sato Ryosuke Kurokawa Rina Sakai Masashi Takaso |
| author_facet | Takuya Yamada Kentaro Uchida Kenji Onuma Jun Kuzuno Masanobu Ujihira Gen Inoue Bunpei Sato Ryosuke Kurokawa Rina Sakai Masashi Takaso |
| author_sort | Takuya Yamada |
| collection | DOAJ |
| description | Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco’s Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting. |
| format | Article |
| id | doaj-art-52e45f3fd1d347efae6187c1b436bd7e |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-52e45f3fd1d347efae6187c1b436bd7e2025-02-03T01:33:23ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/109876109876Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral GraftsTakuya Yamada0Kentaro Uchida1Kenji Onuma2Jun Kuzuno3Masanobu Ujihira4Gen Inoue5Bunpei Sato6Ryosuke Kurokawa7Rina Sakai8Masashi Takaso9Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanMiZ Co., Ltd., 1-16-5 Zenko, Fujisawa, Kanagawa 251-0871, JapanMiZ Co., Ltd., 1-16-5 Zenko, Fujisawa, Kanagawa 251-0871, JapanDepartment of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, JapanAllogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco’s Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting.http://dx.doi.org/10.1155/2014/109876 |
| spellingShingle | Takuya Yamada Kentaro Uchida Kenji Onuma Jun Kuzuno Masanobu Ujihira Gen Inoue Bunpei Sato Ryosuke Kurokawa Rina Sakai Masashi Takaso Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts The Scientific World Journal |
| title | Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts |
| title_full | Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts |
| title_fullStr | Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts |
| title_full_unstemmed | Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts |
| title_short | Hydrogen Supplementation of Preservation Solution Improves Viability of Osteochondral Grafts |
| title_sort | hydrogen supplementation of preservation solution improves viability of osteochondral grafts |
| url | http://dx.doi.org/10.1155/2014/109876 |
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