A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery
The skin graft is a surgical technique commonly used in the reconstructive surgery of the limbs, in order to repair skin loss, as well as to repair the donor area of the flaps and cover the dermal substitutes after engraftment. The unavoidable side effect of this technique consists of unaesthetic sc...
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5043518 |
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| author | Michele Riccio Andrea Marchesini Nicola Zingaretti Sara Carella Letizia Senesi Maria Giuseppina Onesti Pier Camillo Parodi Diego Ribuffo Luca Vaienti Francesco De Francesco |
| author_facet | Michele Riccio Andrea Marchesini Nicola Zingaretti Sara Carella Letizia Senesi Maria Giuseppina Onesti Pier Camillo Parodi Diego Ribuffo Luca Vaienti Francesco De Francesco |
| author_sort | Michele Riccio |
| collection | DOAJ |
| description | The skin graft is a surgical technique commonly used in the reconstructive surgery of the limbs, in order to repair skin loss, as well as to repair the donor area of the flaps and cover the dermal substitutes after engraftment. The unavoidable side effect of this technique consists of unaesthetic scars. In order to achieve the healing of posttraumatic ulcers by means of tissue regeneration and to avoid excessive scarring, a new innovative technology based on the application of autologous micrografts, obtained by Rigenera technology, was reported. This technology was able to induce tissue repair by highly viable skin micrografts of 80 micron size achieved by a mechanical disaggregation method. The specific cell population of these micrografts includes progenitor cells, which in association with the fragment of the Extracellular Matrix (ECM) and growth factors derived by patients’ own tissue initiate biological processes of regeneration enhancing the wound healing process. We have used this technique in 70 cases of traumatic wounds of the lower and upper limbs, characterized by extensive loss of skin substance and soft tissue. In all cases, we have applied the Rigenera protocol using skin micrografts, achieving in 69 cases the complete healing of wounds in a period between 35 and 84 days. For each patient, the reconstructive outcome was evaluated weekly to assess the efficacy of this technique and any arising complication. A visual analogue scale (VAS) was administered to assess the amount of pain felt after the micrografts’ application, whereas we evaluated the scars according to the Vancouver scale and the wound prognosis according to Wound Bed Score. We have thus been able to demonstrate that Rigenera procedure is very effective in stimulating skin regeneration, while reducing the outcome scar. |
| format | Article |
| id | doaj-art-52c32b4dea854145a0585e3827957c6f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-52c32b4dea854145a0585e3827957c6f2025-02-03T05:50:08ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/50435185043518A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative SurgeryMichele Riccio0Andrea Marchesini1Nicola Zingaretti2Sara Carella3Letizia Senesi4Maria Giuseppina Onesti5Pier Camillo Parodi6Diego Ribuffo7Luca Vaienti8Francesco De Francesco9Department of Reconstructive Surgery and Hand Surgery, University Hospital (AOU Ospedali Riuniti di Ancona), via Conca 71, 60123 Torrette di Ancona, Ancona, ItalyDepartment of Reconstructive Surgery and Hand Surgery, University Hospital (AOU Ospedali Riuniti di Ancona), via Conca 71, 60123 Torrette di Ancona, Ancona, ItalyDepartment of Plastic and Reconstructive Surgery, Azienda Ospedaliero-Universitaria “Santa Maria della Misericordia”, Piazzale S. Maria della Misericordia 15, 33100 Udine, ItalyDepartment of Surgery “P.Valdoni”, Unit of Plastic and Reconstructive Surgery, Sapienza University of Rome, Policlinico Umberto I, Rome, ItalyDepartment of Reconstructive Surgery and Hand Surgery, University Hospital (AOU Ospedali Riuniti di Ancona), via Conca 71, 60123 Torrette di Ancona, Ancona, ItalyDepartment of Surgery “P.Valdoni”, Unit of Plastic and Reconstructive Surgery, Sapienza University of Rome, Policlinico Umberto I, Rome, ItalyDepartment of Plastic and Reconstructive Surgery, Azienda Ospedaliero-Universitaria “Santa Maria della Misericordia”, Piazzale S. Maria della Misericordia 15, 33100 Udine, ItalyDepartment of Surgery “P.Valdoni”, Unit of Plastic and Reconstructive Surgery, Sapienza University of Rome, Policlinico Umberto I, Rome, ItalyDepartment of Plastic and Reconstructive Surgery, I.R.C.C.S. Policlinico San Donato, Università degli Studi di Milano, Via Morandi 30, 20097 San Donato Milanese, Milan, ItalyDepartment of Reconstructive Surgery and Hand Surgery, University Hospital (AOU Ospedali Riuniti di Ancona), via Conca 71, 60123 Torrette di Ancona, Ancona, ItalyThe skin graft is a surgical technique commonly used in the reconstructive surgery of the limbs, in order to repair skin loss, as well as to repair the donor area of the flaps and cover the dermal substitutes after engraftment. The unavoidable side effect of this technique consists of unaesthetic scars. In order to achieve the healing of posttraumatic ulcers by means of tissue regeneration and to avoid excessive scarring, a new innovative technology based on the application of autologous micrografts, obtained by Rigenera technology, was reported. This technology was able to induce tissue repair by highly viable skin micrografts of 80 micron size achieved by a mechanical disaggregation method. The specific cell population of these micrografts includes progenitor cells, which in association with the fragment of the Extracellular Matrix (ECM) and growth factors derived by patients’ own tissue initiate biological processes of regeneration enhancing the wound healing process. We have used this technique in 70 cases of traumatic wounds of the lower and upper limbs, characterized by extensive loss of skin substance and soft tissue. In all cases, we have applied the Rigenera protocol using skin micrografts, achieving in 69 cases the complete healing of wounds in a period between 35 and 84 days. For each patient, the reconstructive outcome was evaluated weekly to assess the efficacy of this technique and any arising complication. A visual analogue scale (VAS) was administered to assess the amount of pain felt after the micrografts’ application, whereas we evaluated the scars according to the Vancouver scale and the wound prognosis according to Wound Bed Score. We have thus been able to demonstrate that Rigenera procedure is very effective in stimulating skin regeneration, while reducing the outcome scar.http://dx.doi.org/10.1155/2019/5043518 |
| spellingShingle | Michele Riccio Andrea Marchesini Nicola Zingaretti Sara Carella Letizia Senesi Maria Giuseppina Onesti Pier Camillo Parodi Diego Ribuffo Luca Vaienti Francesco De Francesco A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery Stem Cells International |
| title | A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery |
| title_full | A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery |
| title_fullStr | A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery |
| title_full_unstemmed | A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery |
| title_short | A Multicentre Study: The Use of Micrografts in the Reconstruction of Full-Thickness Posttraumatic Skin Defects of the Limbs—A Whole Innovative Concept in Regenerative Surgery |
| title_sort | multicentre study the use of micrografts in the reconstruction of full thickness posttraumatic skin defects of the limbs a whole innovative concept in regenerative surgery |
| url | http://dx.doi.org/10.1155/2019/5043518 |
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