Preparation of rabbit kidney immortalized cell culture
Preparation of immortalized cell lines obtained from organs and tissues of farm animals is an essential area of biotechnology. The paper presents results of continuous (immortalized) cell line preparation from a primary trypsinized cell culture of an adult rabbit kidney. Cytomorphologic analysis and...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2021-01-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/520 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832086726464503808 |
|---|---|
| author | V. L. Manin I. V. Vologina Ye. A. Trofimova |
| author_facet | V. L. Manin I. V. Vologina Ye. A. Trofimova |
| author_sort | V. L. Manin |
| collection | DOAJ |
| description | Preparation of immortalized cell lines obtained from organs and tissues of farm animals is an essential area of biotechnology. The paper presents results of continuous (immortalized) cell line preparation from a primary trypsinized cell culture of an adult rabbit kidney. Cytomorphologic analysis and karyotyping were performed during the process of subcultivation in the cell culture at passages 1, 3, 24, 31, 38, 56, 66, 75, 86, 101. Dynamics of spontaneous continuous cell line formation during long-term serial passaging was examined using standard nutrient media and fetal serum. Contrary to the known cell lines of rabbit origin (Oryctolagus cuniculus L.), immortalization was not accompanied with enhanced cell production and cell size reduction. The prepared continuous cell line in its adhesive phase was up to 200 µm in size and its productivity was about 7,000 cells/cm2. Significant differences (compared to the known cell lines) in the karyotype were detected during passaging. The formed genotype was found to be near-tetrapioid when the CCL cultural properties were stabilized at passages 66–101. The known cell lines – rabbit kidney (RK-13) and rabbit cornea (SIRC) – can be characterized as pseudotriploid basing on their karyotype. This culture demonstrated low sensitivity to viruses – causative agents of rabbit diseases and sensitivity to heterologous porcine and bovine viruses. |
| format | Article |
| id | doaj-art-525f902df4cb41b3a8a44d93034694c5 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-525f902df4cb41b3a8a44d93034694c52025-02-06T09:52:08ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-01-010429730310.29326/2304-196X-2020-4-35-298-303485Preparation of rabbit kidney immortalized cell cultureV. L. Manin0I. V. Vologina1Ye. A. Trofimova2FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)Preparation of immortalized cell lines obtained from organs and tissues of farm animals is an essential area of biotechnology. The paper presents results of continuous (immortalized) cell line preparation from a primary trypsinized cell culture of an adult rabbit kidney. Cytomorphologic analysis and karyotyping were performed during the process of subcultivation in the cell culture at passages 1, 3, 24, 31, 38, 56, 66, 75, 86, 101. Dynamics of spontaneous continuous cell line formation during long-term serial passaging was examined using standard nutrient media and fetal serum. Contrary to the known cell lines of rabbit origin (Oryctolagus cuniculus L.), immortalization was not accompanied with enhanced cell production and cell size reduction. The prepared continuous cell line in its adhesive phase was up to 200 µm in size and its productivity was about 7,000 cells/cm2. Significant differences (compared to the known cell lines) in the karyotype were detected during passaging. The formed genotype was found to be near-tetrapioid when the CCL cultural properties were stabilized at passages 66–101. The known cell lines – rabbit kidney (RK-13) and rabbit cornea (SIRC) – can be characterized as pseudotriploid basing on their karyotype. This culture demonstrated low sensitivity to viruses – causative agents of rabbit diseases and sensitivity to heterologous porcine and bovine viruses.https://veterinary.arriah.ru/jour/article/view/520continuous cell lineprimary trypsynized cell cultureimmortalizationapoptosistelomere |
| spellingShingle | V. L. Manin I. V. Vologina Ye. A. Trofimova Preparation of rabbit kidney immortalized cell culture Ветеринария сегодня continuous cell line primary trypsynized cell culture immortalization apoptosis telomere |
| title | Preparation of rabbit kidney immortalized cell culture |
| title_full | Preparation of rabbit kidney immortalized cell culture |
| title_fullStr | Preparation of rabbit kidney immortalized cell culture |
| title_full_unstemmed | Preparation of rabbit kidney immortalized cell culture |
| title_short | Preparation of rabbit kidney immortalized cell culture |
| title_sort | preparation of rabbit kidney immortalized cell culture |
| topic | continuous cell line primary trypsynized cell culture immortalization apoptosis telomere |
| url | https://veterinary.arriah.ru/jour/article/view/520 |
| work_keys_str_mv | AT vlmanin preparationofrabbitkidneyimmortalizedcellculture AT ivvologina preparationofrabbitkidneyimmortalizedcellculture AT yeatrofimova preparationofrabbitkidneyimmortalizedcellculture |