Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles

Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles

Summary: Hematopoietic stem cells (HSCs) maintain their self-renewal capacity in an autocrine manner through hematopoietic stem and progenitor cell (HSPC)-derived extracellular vesicles (EVs). Here, we present a protocol for the isolation and characterization of EVs from HSPCs starting from an in vi...

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Bibliographic Details
Main Authors: Federica Zanotti, Ilaria Zanolla, Massimo Bonora, Claudia Morganti, Barbara Zavan, Letizia Ferroni, Paolo Pinton, Keisuke Ito
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Flow Cytometry
Metabolism
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001844
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http://www.sciencedirect.com/science/article/pii/S2666166725001844

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