Expression and Significance of AQP3 in Cutaneous Lesions
Aquaporin 3 (AQP3) is the membrane channel of water and involved in fluid homeostasis. The aim of this study was to reveal the expression and significance of AQP3 in cutaneous lesions. We analyzed AQP3 mRNA levels using RT-PCR in 311 cutaneous lesions and confirmed AQP3 expression in these lesions b...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2021/7866471 |
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| author | Dongfeng Niu Yanhua Bai Qian Yao Wei Hou Lixin Zhou Xiaozheng Huang Chen Zhao |
| author_facet | Dongfeng Niu Yanhua Bai Qian Yao Wei Hou Lixin Zhou Xiaozheng Huang Chen Zhao |
| author_sort | Dongfeng Niu |
| collection | DOAJ |
| description | Aquaporin 3 (AQP3) is the membrane channel of water and involved in fluid homeostasis. The aim of this study was to reveal the expression and significance of AQP3 in cutaneous lesions. We analyzed AQP3 mRNA levels using RT-PCR in 311 cutaneous lesions and confirmed AQP3 expression in these lesions by immunohistochemistry. AQP3 mRNA was detected in normal epidermis, seborrheic keratosis, solar keratosis, Bowen’s disease, squamous cell carcinoma, eccrine poroma, apocrine carcinoma, and sebaceoma; however, AQP3 mRNA was absent in basal cell carcinoma, nevocellular nevus, or malignant melanoma. By immunohistochemistry, diffuse AQP3 expression was seen in all keratotic lesions including seborrheic keratosis, verruca vulgaris, molluscum contagiosum, solar keratosis, Bowen’s disease, and squamous cell carcinoma. Diffuse AQP3 expression was also present in all extramammary Paget’s disease. No AQP3 staining was obtained in basal cell carcinoma. Positive AQP3 staining was seen in sweat gland tumors including hidradenoma, eccrine poroma, and apocrine carcinoma. Among sebaceous tumors, AQP3 expressed diffusely in all sebaceous hyperplasia and sebaceous adenoma, but not in sebaceous carcinomas. Only focal AQP3 staining was seen in nevocellular nevus and no AQP3 staining in melanoma. Our findings indicate the function of AQP3 maintained in most skin tumors. AQP3 may be used for differential diagnosis in skin tumors. |
| format | Article |
| id | doaj-art-514a5c20d05441e8a963716fa49cc620 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-514a5c20d05441e8a963716fa49cc6202025-02-03T01:25:49ZengWileyAnalytical Cellular Pathology2210-71852021-01-01202110.1155/2021/7866471Expression and Significance of AQP3 in Cutaneous LesionsDongfeng Niu0Yanhua Bai1Qian Yao2Wei Hou3Lixin Zhou4Xiaozheng Huang5Chen Zhao6Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Aquaporin 3 (AQP3) is the membrane channel of water and involved in fluid homeostasis. The aim of this study was to reveal the expression and significance of AQP3 in cutaneous lesions. We analyzed AQP3 mRNA levels using RT-PCR in 311 cutaneous lesions and confirmed AQP3 expression in these lesions by immunohistochemistry. AQP3 mRNA was detected in normal epidermis, seborrheic keratosis, solar keratosis, Bowen’s disease, squamous cell carcinoma, eccrine poroma, apocrine carcinoma, and sebaceoma; however, AQP3 mRNA was absent in basal cell carcinoma, nevocellular nevus, or malignant melanoma. By immunohistochemistry, diffuse AQP3 expression was seen in all keratotic lesions including seborrheic keratosis, verruca vulgaris, molluscum contagiosum, solar keratosis, Bowen’s disease, and squamous cell carcinoma. Diffuse AQP3 expression was also present in all extramammary Paget’s disease. No AQP3 staining was obtained in basal cell carcinoma. Positive AQP3 staining was seen in sweat gland tumors including hidradenoma, eccrine poroma, and apocrine carcinoma. Among sebaceous tumors, AQP3 expressed diffusely in all sebaceous hyperplasia and sebaceous adenoma, but not in sebaceous carcinomas. Only focal AQP3 staining was seen in nevocellular nevus and no AQP3 staining in melanoma. Our findings indicate the function of AQP3 maintained in most skin tumors. AQP3 may be used for differential diagnosis in skin tumors.http://dx.doi.org/10.1155/2021/7866471 |
| spellingShingle | Dongfeng Niu Yanhua Bai Qian Yao Wei Hou Lixin Zhou Xiaozheng Huang Chen Zhao Expression and Significance of AQP3 in Cutaneous Lesions Analytical Cellular Pathology |
| title | Expression and Significance of AQP3 in Cutaneous Lesions |
| title_full | Expression and Significance of AQP3 in Cutaneous Lesions |
| title_fullStr | Expression and Significance of AQP3 in Cutaneous Lesions |
| title_full_unstemmed | Expression and Significance of AQP3 in Cutaneous Lesions |
| title_short | Expression and Significance of AQP3 in Cutaneous Lesions |
| title_sort | expression and significance of aqp3 in cutaneous lesions |
| url | http://dx.doi.org/10.1155/2021/7866471 |
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