Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime

Membrane potential (MP) changes can provide a simple readout of bacterial functional and metabolic state or stress levels. While several optical methods exist for measuring fast changes in MP in excitable cells, there is a dearth of such methods for absolute and precise measurements of steady-state...

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Bibliographic Details
Main Authors: Debjit Roy, Xavier Michalet, Evan W. Miller, Kiran Bharadwaj, Shimon Weiss
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Biophysical Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2667074725000011
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