The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis
BackgroundRegulators of n6-methyladenosine (m6A) RNA modification play important roles in many diseases; however, their involvement in Staphylococcus aureus (S. aureus)-related osteomyelitis remains inadequately explored. Therefore, this study aims to investigate the role of m6A in S. aureus infecti...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1526475/full |
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| author | Sijing Liu Sijing Liu Sijing Liu Kai Li Changhai Long Mingwu Lao Biao Ma Changquan Liu Haoyuan He Chunjiang Wang Wangzhu Chen Bin Yu |
| author_facet | Sijing Liu Sijing Liu Sijing Liu Kai Li Changhai Long Mingwu Lao Biao Ma Changquan Liu Haoyuan He Chunjiang Wang Wangzhu Chen Bin Yu |
| author_sort | Sijing Liu |
| collection | DOAJ |
| description | BackgroundRegulators of n6-methyladenosine (m6A) RNA modification play important roles in many diseases; however, their involvement in Staphylococcus aureus (S. aureus)-related osteomyelitis remains inadequately explored. Therefore, this study aims to investigate the role of m6A in S. aureus infection-related osteomyelitis and elucidate its underlying mechanisms.MethodsWe downloaded the S. aureus infection-related osteomyelitis infection dataset GSE30119 from the Gene Expression Omnibus database. Initially, we constructed a diagnostic model based on m6A genes and predicted the hub node miRNAs and transcription factors by constructing a protein–protein interaction network. Subsequently, a prognostic model was built using LASSO regression, the receiver operating characteristic curve of the model was plotted, and the predictive performance of the diagnostic model was validated. Further, unsupervised clustering analysis, gene set enrichment analysis (GSEA), and gene set variation analysis (GSVA) were employed to assess immune cell infiltration. Additionally, we validated the expression of fat mass and obesity-associated protein (FTO) in S. aureus-infected Raw264.7 macrophages using qPCR and western blotting. Moreover, we conducted si-FTO experiments on mouse Raw264.7 macrophages to investigate the anti-inflammatory regulatory role of si-FTO during S. aureus infection.ResultsWe identified 19 co-expressed genes closely related to FTO were identified, along with 206 related transcription factor regulatory genes and 589 miRNAs. Enrichment analyses suggested that these genes were involved in pathways related to the proliferation and oxidation of various immune cells, cellular senescence, and various tumors and immune cells, as well as cell cycle-related functions. GSEA revealed that PD-1, TH1, TH2, CTLA4, and other pathways were significantly enriched in patients with high FTO expression. GSVA indicated that the differentially enriched pathways were related to included amino acid metabolism, immunity, and infection. Correlation analysis of immune infiltration revealed that monocytes, M2 macrophages, resting mast cells, and neutrophils were present in normal and diseased samples. Differences in expression were observed between the groups. The western blotting and qPCR analyses confirmed that the protein expression of FTO was reduced in macrophages after infection with S. aureus, consistent with the observed changes in mRNA expression. Furthermore, we validated that FTO may influence the regulation of inflammation through the FoxO1/NF-kB pathway.ConclusionThe m6A RNA methylation regulator FTO may serve as a potential diagnostic marker and therapeutic target, involved in the pathogenesis of S. aureus infection-related osteomyelitis. This finding provides new insights into the relationship between FTO-mediated m6A RNA methylation and osteomyelitis. |
| format | Article |
| id | doaj-art-4fbd5d3adae64cb6af2afc16d0e74c12 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-4fbd5d3adae64cb6af2afc16d0e74c122025-02-06T07:09:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15264751526475The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitisSijing Liu0Sijing Liu1Sijing Liu2Kai Li3Changhai Long4Mingwu Lao5Biao Ma6Changquan Liu7Haoyuan He8Chunjiang Wang9Wangzhu Chen10Bin Yu11Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, Maoming Hospital of Guangzhou University of Chinese Medicine, Maoming, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, The Second Hospital Affiliated of Guangdong Medical University, Zhanjiang, ChinaDepartment of Orthopaedic Center, Maoming Hospital of Guangzhou University of Chinese Medicine, Maoming, ChinaDepartment of Orthopaedic Center, Maoming Hospital of Guangzhou University of Chinese Medicine, Maoming, ChinaDepartment of Orthopaedic Center, Maoming Hospital of Guangzhou University of Chinese Medicine, Maoming, ChinaDivision of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaBackgroundRegulators of n6-methyladenosine (m6A) RNA modification play important roles in many diseases; however, their involvement in Staphylococcus aureus (S. aureus)-related osteomyelitis remains inadequately explored. Therefore, this study aims to investigate the role of m6A in S. aureus infection-related osteomyelitis and elucidate its underlying mechanisms.MethodsWe downloaded the S. aureus infection-related osteomyelitis infection dataset GSE30119 from the Gene Expression Omnibus database. Initially, we constructed a diagnostic model based on m6A genes and predicted the hub node miRNAs and transcription factors by constructing a protein–protein interaction network. Subsequently, a prognostic model was built using LASSO regression, the receiver operating characteristic curve of the model was plotted, and the predictive performance of the diagnostic model was validated. Further, unsupervised clustering analysis, gene set enrichment analysis (GSEA), and gene set variation analysis (GSVA) were employed to assess immune cell infiltration. Additionally, we validated the expression of fat mass and obesity-associated protein (FTO) in S. aureus-infected Raw264.7 macrophages using qPCR and western blotting. Moreover, we conducted si-FTO experiments on mouse Raw264.7 macrophages to investigate the anti-inflammatory regulatory role of si-FTO during S. aureus infection.ResultsWe identified 19 co-expressed genes closely related to FTO were identified, along with 206 related transcription factor regulatory genes and 589 miRNAs. Enrichment analyses suggested that these genes were involved in pathways related to the proliferation and oxidation of various immune cells, cellular senescence, and various tumors and immune cells, as well as cell cycle-related functions. GSEA revealed that PD-1, TH1, TH2, CTLA4, and other pathways were significantly enriched in patients with high FTO expression. GSVA indicated that the differentially enriched pathways were related to included amino acid metabolism, immunity, and infection. Correlation analysis of immune infiltration revealed that monocytes, M2 macrophages, resting mast cells, and neutrophils were present in normal and diseased samples. Differences in expression were observed between the groups. The western blotting and qPCR analyses confirmed that the protein expression of FTO was reduced in macrophages after infection with S. aureus, consistent with the observed changes in mRNA expression. Furthermore, we validated that FTO may influence the regulation of inflammation through the FoxO1/NF-kB pathway.ConclusionThe m6A RNA methylation regulator FTO may serve as a potential diagnostic marker and therapeutic target, involved in the pathogenesis of S. aureus infection-related osteomyelitis. This finding provides new insights into the relationship between FTO-mediated m6A RNA methylation and osteomyelitis.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1526475/fullStaphylococcus aureusm6AFTOinfectionGSEA |
| spellingShingle | Sijing Liu Sijing Liu Sijing Liu Kai Li Changhai Long Mingwu Lao Biao Ma Changquan Liu Haoyuan He Chunjiang Wang Wangzhu Chen Bin Yu The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis Frontiers in Microbiology Staphylococcus aureus m6A FTO infection GSEA |
| title | The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis |
| title_full | The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis |
| title_fullStr | The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis |
| title_full_unstemmed | The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis |
| title_short | The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis |
| title_sort | role of fto in m6a rna methylation and immune regulation in staphylococcus aureus infection related osteomyelitis |
| topic | Staphylococcus aureus m6A FTO infection GSEA |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1526475/full |
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