Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method
A stability-indicating photochemical method for the assay of riboflavin (RF) in photodegraded samples and aged vitamin preparations has been developed. It is based on photochemical conversion of RF to lumichrome (LC) in alkaline solution under controlled conditions of light intensity, temperature, p...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/256087 |
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| author | Iqbal Ahmad Syed Haider Abbas Zubair Anwar Muhammad Ali Sheraz Sofia Ahmed Adeel Arsalan Raheela Bano |
| author_facet | Iqbal Ahmad Syed Haider Abbas Zubair Anwar Muhammad Ali Sheraz Sofia Ahmed Adeel Arsalan Raheela Bano |
| author_sort | Iqbal Ahmad |
| collection | DOAJ |
| description | A stability-indicating photochemical method for the assay of riboflavin (RF) in photodegraded samples and aged vitamin preparations has been developed. It is based on photochemical conversion of RF to lumichrome (LC) in alkaline solution under controlled conditions of light intensity, temperature, pH, time of exposure, and distance. Under these conditions about two-thirds of RF is converted to LC and on the basis of the RF : LC ratio the concentration of RF can be determined in degraded solutions. The method involves the extraction of photolyzed solutions of RF (pH 2.0) with chloroform and determination of LC along with lumiflavin (LF) by a two-component spectrometric method at 356 and 445 nm. The method has been validated and the results of the assay of RF in photodegraded solutions compare well with those of the standard USP fluorimetric method. The recovery of the method is 99–101% and the precision is within 2%. The method is stability-indicating and can be applied to the assay of RF in photodegraded solutions and aged vitamin preparations. The method is specific compared to that of the USP fluorimetric method in which the degraded LC may interfere with the fluorescence emission of RF. |
| format | Article |
| id | doaj-art-4f0d1bbe33974691b8aa27ad6c60d02c |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-4f0d1bbe33974691b8aa27ad6c60d02c2025-02-03T05:59:25ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/256087256087Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome MethodIqbal Ahmad0Syed Haider Abbas1Zubair Anwar2Muhammad Ali Sheraz3Sofia Ahmed4Adeel Arsalan5Raheela Bano6Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanBaqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi 74600, PakistanA stability-indicating photochemical method for the assay of riboflavin (RF) in photodegraded samples and aged vitamin preparations has been developed. It is based on photochemical conversion of RF to lumichrome (LC) in alkaline solution under controlled conditions of light intensity, temperature, pH, time of exposure, and distance. Under these conditions about two-thirds of RF is converted to LC and on the basis of the RF : LC ratio the concentration of RF can be determined in degraded solutions. The method involves the extraction of photolyzed solutions of RF (pH 2.0) with chloroform and determination of LC along with lumiflavin (LF) by a two-component spectrometric method at 356 and 445 nm. The method has been validated and the results of the assay of RF in photodegraded solutions compare well with those of the standard USP fluorimetric method. The recovery of the method is 99–101% and the precision is within 2%. The method is stability-indicating and can be applied to the assay of RF in photodegraded solutions and aged vitamin preparations. The method is specific compared to that of the USP fluorimetric method in which the degraded LC may interfere with the fluorescence emission of RF.http://dx.doi.org/10.1155/2015/256087 |
| spellingShingle | Iqbal Ahmad Syed Haider Abbas Zubair Anwar Muhammad Ali Sheraz Sofia Ahmed Adeel Arsalan Raheela Bano Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method Journal of Chemistry |
| title | Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method |
| title_full | Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method |
| title_fullStr | Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method |
| title_full_unstemmed | Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method |
| title_short | Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method |
| title_sort | stability indicating photochemical method for the assay of riboflavin lumichrome method |
| url | http://dx.doi.org/10.1155/2015/256087 |
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