Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi
ABSTRACT The oleaginous yeast Lipomyces starkeyi is recognized for its remarkable lipid accumulation under nitrogen‐limited conditions. However, precise control of microbial lipid production in L. starkeyi remains challenging due to the complexity of nutrient media. We developed a two‐stage fed‐batc...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Engineering in Life Sciences |
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| Online Access: | https://doi.org/10.1002/elsc.70003 |
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| author | Chih‐Chan Wu Kenji Okano Pijar Religia Yuki Soma Masatomo Takahashi Yoshihiro Izumi Takeshi Bamba Kohsuke Honda |
| author_facet | Chih‐Chan Wu Kenji Okano Pijar Religia Yuki Soma Masatomo Takahashi Yoshihiro Izumi Takeshi Bamba Kohsuke Honda |
| author_sort | Chih‐Chan Wu |
| collection | DOAJ |
| description | ABSTRACT The oleaginous yeast Lipomyces starkeyi is recognized for its remarkable lipid accumulation under nitrogen‐limited conditions. However, precise control of microbial lipid production in L. starkeyi remains challenging due to the complexity of nutrient media. We developed a two‐stage fed‐batch fermentation process using a well‐defined synthetic medium in a 5‐L bioreactor. In the first stage, the specific growth rate was maintained at a designated level by maximizing the cell density through optimizing the feeding rate, molar carbon‐to‐nitrogen (C/N) ratio, and phosphate concentration in feeding media, achieving a high cell density of 213 ± 10 × 107 cells mL−1. In the second stage, we optimized the molar C/N ratio in the feeding medium for lipid production and achieved high biomass (130 ± 5 g L−1), lipid titer (88 ± 6 g L−1), and lipid content (67% ± 2% of dry cellular weight). Our approach yielded a high lipid titer, comparable to the highest reported value of 68 g L−1 achieved in a nutrient medium, by optimizing cultivation conditions with a synthetic medium in L. starkeyi. This highlights the importance of well‐established yet powerful bioprocess approaches for the precise control of microbial cultivation. |
| format | Article |
| id | doaj-art-4e986b5ce4f84914b03eee11871cb39c |
| institution | Kabale University |
| issn | 1618-0240 1618-2863 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj-art-4e986b5ce4f84914b03eee11871cb39c2025-01-30T06:40:31ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632025-01-01251n/an/a10.1002/elsc.70003Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyiChih‐Chan Wu0Kenji Okano1Pijar Religia2Yuki Soma3Masatomo Takahashi4Yoshihiro Izumi5Takeshi Bamba6Kohsuke Honda7International Center for Biotechnology Osaka University Suita Osaka JapanInternational Center for Biotechnology Osaka University Suita Osaka JapanInternational Center for Biotechnology Osaka University Suita Osaka JapanSynthetic Bioengineering Research Group Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology Tsukuba Ibaraki JapanDivision of Metabolomics/Mass Spectrometry Center Medical Research Center for High Depth Omics Medical Institute of Bioregulation Kyushu University Fukuoka JapanDivision of Metabolomics/Mass Spectrometry Center Medical Research Center for High Depth Omics Medical Institute of Bioregulation Kyushu University Fukuoka JapanDivision of Metabolomics/Mass Spectrometry Center Medical Research Center for High Depth Omics Medical Institute of Bioregulation Kyushu University Fukuoka JapanInternational Center for Biotechnology Osaka University Suita Osaka JapanABSTRACT The oleaginous yeast Lipomyces starkeyi is recognized for its remarkable lipid accumulation under nitrogen‐limited conditions. However, precise control of microbial lipid production in L. starkeyi remains challenging due to the complexity of nutrient media. We developed a two‐stage fed‐batch fermentation process using a well‐defined synthetic medium in a 5‐L bioreactor. In the first stage, the specific growth rate was maintained at a designated level by maximizing the cell density through optimizing the feeding rate, molar carbon‐to‐nitrogen (C/N) ratio, and phosphate concentration in feeding media, achieving a high cell density of 213 ± 10 × 107 cells mL−1. In the second stage, we optimized the molar C/N ratio in the feeding medium for lipid production and achieved high biomass (130 ± 5 g L−1), lipid titer (88 ± 6 g L−1), and lipid content (67% ± 2% of dry cellular weight). Our approach yielded a high lipid titer, comparable to the highest reported value of 68 g L−1 achieved in a nutrient medium, by optimizing cultivation conditions with a synthetic medium in L. starkeyi. This highlights the importance of well‐established yet powerful bioprocess approaches for the precise control of microbial cultivation.https://doi.org/10.1002/elsc.70003fermentationhigh cell densityLipomyces starkeyimicrobial lipidoleaginous yeastsynthetic medium |
| spellingShingle | Chih‐Chan Wu Kenji Okano Pijar Religia Yuki Soma Masatomo Takahashi Yoshihiro Izumi Takeshi Bamba Kohsuke Honda Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi Engineering in Life Sciences fermentation high cell density Lipomyces starkeyi microbial lipid oleaginous yeast synthetic medium |
| title | Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi |
| title_full | Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi |
| title_fullStr | Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi |
| title_full_unstemmed | Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi |
| title_short | Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi |
| title_sort | combination of two stage continuous feeding and optimized synthetic medium increases lipid production in lipomyces starkeyi |
| topic | fermentation high cell density Lipomyces starkeyi microbial lipid oleaginous yeast synthetic medium |
| url | https://doi.org/10.1002/elsc.70003 |
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