Structural and Functional Annotation of Hypothetical Proteins of O139
In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of vari...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2015-06-01
|
| Series: | Genomics & Informatics |
| Subjects: | |
| Online Access: | http://genominfo.org/upload/pdf/gni-13-53.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832569520183574528 |
|---|---|
| author | Md. Saiful Islam Shah Md. Shahik Md. Sohel Noman I. A. Patwary Md. Anayet Hasan |
| author_facet | Md. Saiful Islam Shah Md. Shahik Md. Sohel Noman I. A. Patwary Md. Anayet Hasan |
| author_sort | Md. Saiful Islam |
| collection | DOAJ |
| description | In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera. |
| format | Article |
| id | doaj-art-4e4b001ac85441faa64d6bcf2a87641c |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-4e4b001ac85441faa64d6bcf2a87641c2025-02-02T20:37:56ZengBioMed CentralGenomics & Informatics1598-866X2234-07422015-06-01132535910.5808/GI.2015.13.2.53153Structural and Functional Annotation of Hypothetical Proteins of O139Md. Saiful Islam0Shah Md. Shahik1Md. Sohel2Noman I. A. Patwary3Md. Anayet Hasan4Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera.http://genominfo.org/upload/pdf/gni-13-53.pdfcholeracomputational toolsdockingdrug discovery O139 |
| spellingShingle | Md. Saiful Islam Shah Md. Shahik Md. Sohel Noman I. A. Patwary Md. Anayet Hasan Structural and Functional Annotation of Hypothetical Proteins of O139 Genomics & Informatics cholera computational tools docking drug discovery O139 |
| title | Structural and Functional Annotation of Hypothetical Proteins of O139 |
| title_full | Structural and Functional Annotation of Hypothetical Proteins of O139 |
| title_fullStr | Structural and Functional Annotation of Hypothetical Proteins of O139 |
| title_full_unstemmed | Structural and Functional Annotation of Hypothetical Proteins of O139 |
| title_short | Structural and Functional Annotation of Hypothetical Proteins of O139 |
| title_sort | structural and functional annotation of hypothetical proteins of o139 |
| topic | cholera computational tools docking drug discovery O139 |
| url | http://genominfo.org/upload/pdf/gni-13-53.pdf |
| work_keys_str_mv | AT mdsaifulislam structuralandfunctionalannotationofhypotheticalproteinsofo139 AT shahmdshahik structuralandfunctionalannotationofhypotheticalproteinsofo139 AT mdsohel structuralandfunctionalannotationofhypotheticalproteinsofo139 AT nomaniapatwary structuralandfunctionalannotationofhypotheticalproteinsofo139 AT mdanayethasan structuralandfunctionalannotationofhypotheticalproteinsofo139 |