Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt
<i>Paenibacillus polymyxa</i> strain PJH16, isolated and tested by our team, suppresses cucumber <i>Fusarium</i> wilt as an efficient biocontrol agent. For further investigation, the strain has been combined with two other <i>Bacillus strains</i> (<i>Bacillu...
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2025-01-01
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| author | Fan Yang Xin Wang Huayan Jiang Xiaoke Chang Weiwei Chen Gongyao Shi Baoming Tian Qiuju Yao |
| author_facet | Fan Yang Xin Wang Huayan Jiang Xiaoke Chang Weiwei Chen Gongyao Shi Baoming Tian Qiuju Yao |
| author_sort | Fan Yang |
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| description | <i>Paenibacillus polymyxa</i> strain PJH16, isolated and tested by our team, suppresses cucumber <i>Fusarium</i> wilt as an efficient biocontrol agent. For further investigation, the strain has been combined with two other <i>Bacillus strains</i> (<i>Bacillus velezensis</i> VJH504 and <i>Bacillus subtilis</i> JNF2) to enhance biocontrol ability, which formed high-efficiency microbial agents in the current study. The methodological target taken is based on achieving the optimal growth conditions of the combined microbial agents; hence, the medium composition and culture conditions were optimized through a single-factor test, orthogonal test and response surface methodology. Following this, the effectiveness of the microbial combination was assessed through pot experiments, which provided a theoretical foundation for the synthesis of microbial flora to significantly control cucumber Fusarium wilt. The results showed excellent compatibility, proving suitable for the proliferation and growth of <i>Paenibacillus polymyxa</i> PJH16, <i>Bacillus velezensis</i> VJH504, and <i>Bacillus subtilis</i> JNF2 strains together, specifically, when the inoculation amounts were adjusted to 4% of each. Using the single-factor test and orthogonal test analysis, the optimum composition of culture medium for the composite strain was identified as 3% glucose as the optimal carbon source, 2% yeast extract powder as the preferred nitrogen source, and 1% dipotassium hydrogen phosphate as the most suitable inorganic salt. Furthermore, the optical density (OD<sub>600</sub>) of the composite strain solution reached its highest level at 3.16 under the following culture conditions: inoculation volume of 200 µL, 171 rpm culture speed, 21.6 h culture time, 30 °C cultural temperature, and an initial pH of 7.0. The pot experiment demonstrated that the mixed bacterial solution achieved a relative control efficacy of 93.4% against cucumber <i>Fusarium</i> wilt, which was significantly superior to that of single- strain or pesticide treatment, and also promoted cucumber growth. In summary, the microbial flora synthesized by the three <i>Bacillus</i> strains displayed a high bacterial concentration, following the optimization of culture conditions, and exerted remarkable control and growth-promoting effects on cucumber <i>Fusarium</i> wilt. This finding holds great significance for future developments of composite microbial agents. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-4e1ab54c093947c9b7d3beac1b12591f2025-01-24T13:42:46ZengMDPI AGMicroorganisms2076-26072025-01-0113113310.3390/microorganisms13010133Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> WiltFan Yang0Xin Wang1Huayan Jiang2Xiaoke Chang3Weiwei Chen4Gongyao Shi5Baoming Tian6Qiuju Yao7Institute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaInstitute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China<i>Paenibacillus polymyxa</i> strain PJH16, isolated and tested by our team, suppresses cucumber <i>Fusarium</i> wilt as an efficient biocontrol agent. For further investigation, the strain has been combined with two other <i>Bacillus strains</i> (<i>Bacillus velezensis</i> VJH504 and <i>Bacillus subtilis</i> JNF2) to enhance biocontrol ability, which formed high-efficiency microbial agents in the current study. The methodological target taken is based on achieving the optimal growth conditions of the combined microbial agents; hence, the medium composition and culture conditions were optimized through a single-factor test, orthogonal test and response surface methodology. Following this, the effectiveness of the microbial combination was assessed through pot experiments, which provided a theoretical foundation for the synthesis of microbial flora to significantly control cucumber Fusarium wilt. The results showed excellent compatibility, proving suitable for the proliferation and growth of <i>Paenibacillus polymyxa</i> PJH16, <i>Bacillus velezensis</i> VJH504, and <i>Bacillus subtilis</i> JNF2 strains together, specifically, when the inoculation amounts were adjusted to 4% of each. Using the single-factor test and orthogonal test analysis, the optimum composition of culture medium for the composite strain was identified as 3% glucose as the optimal carbon source, 2% yeast extract powder as the preferred nitrogen source, and 1% dipotassium hydrogen phosphate as the most suitable inorganic salt. Furthermore, the optical density (OD<sub>600</sub>) of the composite strain solution reached its highest level at 3.16 under the following culture conditions: inoculation volume of 200 µL, 171 rpm culture speed, 21.6 h culture time, 30 °C cultural temperature, and an initial pH of 7.0. The pot experiment demonstrated that the mixed bacterial solution achieved a relative control efficacy of 93.4% against cucumber <i>Fusarium</i> wilt, which was significantly superior to that of single- strain or pesticide treatment, and also promoted cucumber growth. In summary, the microbial flora synthesized by the three <i>Bacillus</i> strains displayed a high bacterial concentration, following the optimization of culture conditions, and exerted remarkable control and growth-promoting effects on cucumber <i>Fusarium</i> wilt. This finding holds great significance for future developments of composite microbial agents.https://www.mdpi.com/2076-2607/13/1/133synthetic microbial communitysingle-factor testorthogonal testresponse surface methodcucumber <i>Fusarium</i> wilt |
| spellingShingle | Fan Yang Xin Wang Huayan Jiang Xiaoke Chang Weiwei Chen Gongyao Shi Baoming Tian Qiuju Yao Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt Microorganisms synthetic microbial community single-factor test orthogonal test response surface method cucumber <i>Fusarium</i> wilt |
| title | Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt |
| title_full | Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt |
| title_fullStr | Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt |
| title_full_unstemmed | Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt |
| title_short | Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber <i>Fusarium</i> Wilt |
| title_sort | formation of a novel antagonistic bacterial combination to enhance biocontrol for cucumber i fusarium i wilt |
| topic | synthetic microbial community single-factor test orthogonal test response surface method cucumber <i>Fusarium</i> wilt |
| url | https://www.mdpi.com/2076-2607/13/1/133 |
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