Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels
Introduction. Management of phenylketonuria (PKU) is achieved through low-phenylalanine (Phe) diet, supplemented with low-protein food and mixture of free-synthetic (FS) amino acid (AA). Casein glycomacropeptide (CGMP) is a natural peptide released in whey during cheese-making and does not contain P...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2018/6352919 |
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| author | Kirsten K. Ahring Allan M. Lund Erik Jensen Thomas G. Jensen Karen Brøndum-Nielsen Michael Pedersen Allan Bardow Jens Juul Holst Jens F. Rehfeld Lisbeth B. Møller |
| author_facet | Kirsten K. Ahring Allan M. Lund Erik Jensen Thomas G. Jensen Karen Brøndum-Nielsen Michael Pedersen Allan Bardow Jens Juul Holst Jens F. Rehfeld Lisbeth B. Møller |
| author_sort | Kirsten K. Ahring |
| collection | DOAJ |
| description | Introduction. Management of phenylketonuria (PKU) is achieved through low-phenylalanine (Phe) diet, supplemented with low-protein food and mixture of free-synthetic (FS) amino acid (AA). Casein glycomacropeptide (CGMP) is a natural peptide released in whey during cheese-making and does not contain Phe. Lacprodan® CGMP-20 used in this study contained a small amount of Phe due to minor presence of other proteins/peptides. Objective. The purpose of this study was to compare absorption of CGMP-20 to FSAA with the aim of evaluating short-term effects on plasma AAs as well as biomarkers related to food intake. Methods. This study included 8 patients, who had four visits and tested four drink mixtures (DM1–4), consisting of CGMP, FSAA, or a combination. Plasma blood samples were collected at baseline, 15, 30, 60, 120, and 240 minutes (min) after the meal. AA profiles and ghrelin were determined 6 times, while surrogate biomarkers were determined at baseline and 240 min. A visual analogue scale (VAS) was used for evaluation of taste and satiety. Results. The surrogate biomarker concentrations and VAS scores for satiety and taste were nonsignificant between the four DMs, and there were only few significant results for AA profiles (not Phe). Conclusion. CGMP and FSAA had the overall same nonsignificant short-term effect on biomarkers, including Phe. This combination of FSAA and CGMP is a suitable supplement for PKU patients. |
| format | Article |
| id | doaj-art-4dd620c5c0d74e229a6660cc019cb87a |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-4dd620c5c0d74e229a6660cc019cb87a2025-02-03T07:24:45ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322018-01-01201810.1155/2018/63529196352919Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe LevelsKirsten K. Ahring0Allan M. Lund1Erik Jensen2Thomas G. Jensen3Karen Brøndum-Nielsen4Michael Pedersen5Allan Bardow6Jens Juul Holst7Jens F. Rehfeld8Lisbeth B. Møller9The PKU Clinic, Kennedy Centre, Centre for Paediatric and Adolescent Medicine, Copenhagen University Hospital, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Genetics, Applied Human Molecular Genetics, Kennedy Center, Rigshospitalet, DenmarkArla Foods Ingredients Group P/S, Viby J, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus, DenmarkThe PKU Clinic, Kennedy Centre, Centre for Paediatric and Adolescent Medicine, Copenhagen University Hospital, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Medicine, Aarhus University Hospital, Aarhus, DenmarkDepartment of Odontology, Copenhagen University, Copenhagen, DenmarkInstitute of Clinical Medicine, Copenhagen University Hospital, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Biochemistry, University of Copenhagen, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Genetics, Applied Human Molecular Genetics, Kennedy Center, Rigshospitalet, DenmarkIntroduction. Management of phenylketonuria (PKU) is achieved through low-phenylalanine (Phe) diet, supplemented with low-protein food and mixture of free-synthetic (FS) amino acid (AA). Casein glycomacropeptide (CGMP) is a natural peptide released in whey during cheese-making and does not contain Phe. Lacprodan® CGMP-20 used in this study contained a small amount of Phe due to minor presence of other proteins/peptides. Objective. The purpose of this study was to compare absorption of CGMP-20 to FSAA with the aim of evaluating short-term effects on plasma AAs as well as biomarkers related to food intake. Methods. This study included 8 patients, who had four visits and tested four drink mixtures (DM1–4), consisting of CGMP, FSAA, or a combination. Plasma blood samples were collected at baseline, 15, 30, 60, 120, and 240 minutes (min) after the meal. AA profiles and ghrelin were determined 6 times, while surrogate biomarkers were determined at baseline and 240 min. A visual analogue scale (VAS) was used for evaluation of taste and satiety. Results. The surrogate biomarker concentrations and VAS scores for satiety and taste were nonsignificant between the four DMs, and there were only few significant results for AA profiles (not Phe). Conclusion. CGMP and FSAA had the overall same nonsignificant short-term effect on biomarkers, including Phe. This combination of FSAA and CGMP is a suitable supplement for PKU patients.http://dx.doi.org/10.1155/2018/6352919 |
| spellingShingle | Kirsten K. Ahring Allan M. Lund Erik Jensen Thomas G. Jensen Karen Brøndum-Nielsen Michael Pedersen Allan Bardow Jens Juul Holst Jens F. Rehfeld Lisbeth B. Møller Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels Journal of Nutrition and Metabolism |
| title | Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels |
| title_full | Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels |
| title_fullStr | Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels |
| title_full_unstemmed | Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels |
| title_short | Comparison of Glycomacropeptide with Phenylalanine Free-Synthetic Amino Acids in Test Meals to PKU Patients: No Significant Differences in Biomarkers, Including Plasma Phe Levels |
| title_sort | comparison of glycomacropeptide with phenylalanine free synthetic amino acids in test meals to pku patients no significant differences in biomarkers including plasma phe levels |
| url | http://dx.doi.org/10.1155/2018/6352919 |
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