A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice
Abstract The emerging of emergent SARS‐CoV‐2 subvariants has reduced the protective efficacy of COVID‐19 vaccines. Therefore, novel COVID‐19 vaccines targeting these emergent variants are needed. We designed and prepared CoV072, an mRNA‐based vaccine against SARS‐CoV‐2 Omicron (EG.5) and other emerg...
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Wiley
2025-01-01
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| Online Access: | https://doi.org/10.1002/mco2.779 |
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| author | Hongyu Wang Qinhua Peng Xinxian Dai Zhifang Ying Xiaohong Wu Xinyu Liu Hongshan Xu Jia Li Leitai Shi Jingjing Liu Yunpeng Wang Danhua Zhao Yanqiu Huang Lihong Yang Ren Yang Guangzhi Yue Yue Suo Qiang Ye Shouchun Cao Yuhua Li |
| author_facet | Hongyu Wang Qinhua Peng Xinxian Dai Zhifang Ying Xiaohong Wu Xinyu Liu Hongshan Xu Jia Li Leitai Shi Jingjing Liu Yunpeng Wang Danhua Zhao Yanqiu Huang Lihong Yang Ren Yang Guangzhi Yue Yue Suo Qiang Ye Shouchun Cao Yuhua Li |
| author_sort | Hongyu Wang |
| collection | DOAJ |
| description | Abstract The emerging of emergent SARS‐CoV‐2 subvariants has reduced the protective efficacy of COVID‐19 vaccines. Therefore, novel COVID‐19 vaccines targeting these emergent variants are needed. We designed and prepared CoV072, an mRNA‐based vaccine against SARS‐CoV‐2 Omicron (EG.5) and other emergent SARS‐CoV‐2 subvariants that encodes the EG.5 spike protein. Six‐week‐old female BALB/C mice were used to assess humoral and cellular immune responses and cross‐reactive neutralizing activity against various SARS‐CoV‐2 subvariants. Meanwhile different immunization strategies and doses were performed to detect the immunogenicity of this mRNA vaccine. Our results show that two doses of 5 µg CoV072 or a single dose of 15 µg CoV072 both induced broad‐spectrum cross‐protection ability in mice. Compared with a single dose of 15 µg CoV072, two doses of 5 µg COV072 exhibited higher levels of pseudovirus neutralizing antibody (PNAb) and cross‐reactive IgG responses to multiple variants. Moreover, higher levels of neutralizing antibody (NAb) against live XBB and EG.5 variants were also induced. Th1‐biased cellular immune response was induced in all vaccination groups. The antigen design and immunization strategy of this study have reference significance for the research of the next generation of COVID‐19 vaccine and other vaccines. |
| format | Article |
| id | doaj-art-4c43c99b442242e7abc53d652e699779 |
| institution | Kabale University |
| issn | 2688-2663 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj-art-4c43c99b442242e7abc53d652e6997792025-01-20T01:45:44ZengWileyMedComm2688-26632025-01-0161n/an/a10.1002/mco2.779A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in miceHongyu Wang0Qinhua Peng1Xinxian Dai2Zhifang Ying3Xiaohong Wu4Xinyu Liu5Hongshan Xu6Jia Li7Leitai Shi8Jingjing Liu9Yunpeng Wang10Danhua Zhao11Yanqiu Huang12Lihong Yang13Ren Yang14Guangzhi Yue15Yue Suo16Qiang Ye17Shouchun Cao18Yuhua Li19Department of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaEtiology Laboratory，National Vaccine and Serum InstituteBeijing ChinaDivision of Respiratory Virus VaccinesNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaDepartment of Arboviral VaccineNational Institutes for Food and Drug ControlBeijingChinaAbstract The emerging of emergent SARS‐CoV‐2 subvariants has reduced the protective efficacy of COVID‐19 vaccines. Therefore, novel COVID‐19 vaccines targeting these emergent variants are needed. We designed and prepared CoV072, an mRNA‐based vaccine against SARS‐CoV‐2 Omicron (EG.5) and other emergent SARS‐CoV‐2 subvariants that encodes the EG.5 spike protein. Six‐week‐old female BALB/C mice were used to assess humoral and cellular immune responses and cross‐reactive neutralizing activity against various SARS‐CoV‐2 subvariants. Meanwhile different immunization strategies and doses were performed to detect the immunogenicity of this mRNA vaccine. Our results show that two doses of 5 µg CoV072 or a single dose of 15 µg CoV072 both induced broad‐spectrum cross‐protection ability in mice. Compared with a single dose of 15 µg CoV072, two doses of 5 µg COV072 exhibited higher levels of pseudovirus neutralizing antibody (PNAb) and cross‐reactive IgG responses to multiple variants. Moreover, higher levels of neutralizing antibody (NAb) against live XBB and EG.5 variants were also induced. Th1‐biased cellular immune response was induced in all vaccination groups. The antigen design and immunization strategy of this study have reference significance for the research of the next generation of COVID‐19 vaccine and other vaccines.https://doi.org/10.1002/mco2.779immunization strategiesimmunogenicitymRNA‐based vaccinesSARS‐CoV‐2 variants |
| spellingShingle | Hongyu Wang Qinhua Peng Xinxian Dai Zhifang Ying Xiaohong Wu Xinyu Liu Hongshan Xu Jia Li Leitai Shi Jingjing Liu Yunpeng Wang Danhua Zhao Yanqiu Huang Lihong Yang Ren Yang Guangzhi Yue Yue Suo Qiang Ye Shouchun Cao Yuhua Li A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice MedComm immunization strategies immunogenicity mRNA‐based vaccines SARS‐CoV‐2 variants |
| title | A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice |
| title_full | A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice |
| title_fullStr | A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice |
| title_full_unstemmed | A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice |
| title_short | A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice |
| title_sort | sars cov 2 eg 5 mrna vaccine induces a broad spectrum immune response in mice |
| topic | immunization strategies immunogenicity mRNA‐based vaccines SARS‐CoV‐2 variants |
| url | https://doi.org/10.1002/mco2.779 |
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