Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis
Background/Objective: We aimed to elucidate the roles of ferroptosis-associated differentially expressed genes (DEGs) in glioblastoma and provide a comprehensive resource for researchers in the field of glioblastoma cell ferroptosis. Methods: We used RNA sequencing to identify the DEGs associated wi...
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2024-12-01
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| author | Zijiang Yang Ting Zhang Xuanlin Zhu Xiaobiao Zhang |
| author_facet | Zijiang Yang Ting Zhang Xuanlin Zhu Xiaobiao Zhang |
| author_sort | Zijiang Yang |
| collection | DOAJ |
| description | Background/Objective: We aimed to elucidate the roles of ferroptosis-associated differentially expressed genes (DEGs) in glioblastoma and provide a comprehensive resource for researchers in the field of glioblastoma cell ferroptosis. Methods: We used RNA sequencing to identify the DEGs associated with erastin-induced ferroptosis in glioblastoma cells. We further unraveled the biological functions and clinical implications of cold-inducible RNA-binding protein (CIRBP) in the context of glioblastoma by using a multifaceted approach, encompassing gene expression profiling, survival analysis, and functional assays to elucidate its role in glioblastoma cell mortality and its potential influence on patient prognosis. Results: We identified and validated the gene encoding CIRBP, the expression of which is altered during glioblastoma ferroptosis. Our findings highlight the relationship between CIRBP expression and ferroptosis in glioblastoma cells. We demonstrated that CIRBP modulates key aspects of cell death, thereby altering the sensitivity of glioblastoma cells to erastin-induced ferroptosis. A prognostic model, constructed based on <i>CIRBP</i> expression levels, revealed an association between lower <i>CIRBP</i> levels and poorer prognosis in glioma patients; this finding was corroborated by our comprehensive in vitro and in vivo assays that highlighted the impact of modulating CIRBP expression on glioblastoma cell viability and ferroptotic response. Conclusion: Our research unravels the complex molecular dynamics of ferroptosis in glioblastoma and underscores CIRBP as a potential biomarker and therapeutic target. This improved understanding of the role of CIRBP in ferroptosis paves the way for more precise and efficacious treatments for glioblastoma, potentially improving patient outcomes. |
| format | Article |
| id | doaj-art-4bd7375ae9df4f908e23f17187deafa8 |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj-art-4bd7375ae9df4f908e23f17187deafa82025-01-24T13:23:49ZengMDPI AGBiomedicines2227-90592024-12-011314110.3390/biomedicines13010041Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells FerroptosisZijiang Yang0Ting Zhang1Xuanlin Zhu2Xiaobiao Zhang3Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Central Laboratory, Jiangyin Clinical College of Xuzhou Medical University, Jiangyin 214400, ChinaSchool of Basic Medical Sciences, Naval Medical University (Second Military Medical University), Shanghai 200433, ChinaDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaBackground/Objective: We aimed to elucidate the roles of ferroptosis-associated differentially expressed genes (DEGs) in glioblastoma and provide a comprehensive resource for researchers in the field of glioblastoma cell ferroptosis. Methods: We used RNA sequencing to identify the DEGs associated with erastin-induced ferroptosis in glioblastoma cells. We further unraveled the biological functions and clinical implications of cold-inducible RNA-binding protein (CIRBP) in the context of glioblastoma by using a multifaceted approach, encompassing gene expression profiling, survival analysis, and functional assays to elucidate its role in glioblastoma cell mortality and its potential influence on patient prognosis. Results: We identified and validated the gene encoding CIRBP, the expression of which is altered during glioblastoma ferroptosis. Our findings highlight the relationship between CIRBP expression and ferroptosis in glioblastoma cells. We demonstrated that CIRBP modulates key aspects of cell death, thereby altering the sensitivity of glioblastoma cells to erastin-induced ferroptosis. A prognostic model, constructed based on <i>CIRBP</i> expression levels, revealed an association between lower <i>CIRBP</i> levels and poorer prognosis in glioma patients; this finding was corroborated by our comprehensive in vitro and in vivo assays that highlighted the impact of modulating CIRBP expression on glioblastoma cell viability and ferroptotic response. Conclusion: Our research unravels the complex molecular dynamics of ferroptosis in glioblastoma and underscores CIRBP as a potential biomarker and therapeutic target. This improved understanding of the role of CIRBP in ferroptosis paves the way for more precise and efficacious treatments for glioblastoma, potentially improving patient outcomes.https://www.mdpi.com/2227-9059/13/1/41ferroptosisglioma progressionlipid peroxidationRNA sequencingcold-inducible RNA-binding proteinendoplasmic reticulum stress |
| spellingShingle | Zijiang Yang Ting Zhang Xuanlin Zhu Xiaobiao Zhang Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis Biomedicines ferroptosis glioma progression lipid peroxidation RNA sequencing cold-inducible RNA-binding protein endoplasmic reticulum stress |
| title | Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis |
| title_full | Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis |
| title_fullStr | Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis |
| title_full_unstemmed | Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis |
| title_short | Ferroptosis-Related Transcriptional Level Changes and the Role of CIRBP in Glioblastoma Cells Ferroptosis |
| title_sort | ferroptosis related transcriptional level changes and the role of cirbp in glioblastoma cells ferroptosis |
| topic | ferroptosis glioma progression lipid peroxidation RNA sequencing cold-inducible RNA-binding protein endoplasmic reticulum stress |
| url | https://www.mdpi.com/2227-9059/13/1/41 |
| work_keys_str_mv | AT zijiangyang ferroptosisrelatedtranscriptionallevelchangesandtheroleofcirbpinglioblastomacellsferroptosis AT tingzhang ferroptosisrelatedtranscriptionallevelchangesandtheroleofcirbpinglioblastomacellsferroptosis AT xuanlinzhu ferroptosisrelatedtranscriptionallevelchangesandtheroleofcirbpinglioblastomacellsferroptosis AT xiaobiaozhang ferroptosisrelatedtranscriptionallevelchangesandtheroleofcirbpinglioblastomacellsferroptosis |