Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin
The antioxidant and metal chelating activities in J. curcas protein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse pha...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/190129 |
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| author | Santiago Gallegos Tintoré Cristina Torres Fuentes Javier Solorza Feria Manuel Alaiz Julio Girón Calle Alma Leticia Martínez Ayala Luis Chel Guerrero Javier Vioque |
| author_facet | Santiago Gallegos Tintoré Cristina Torres Fuentes Javier Solorza Feria Manuel Alaiz Julio Girón Calle Alma Leticia Martínez Ayala Luis Chel Guerrero Javier Vioque |
| author_sort | Santiago Gallegos Tintoré |
| collection | DOAJ |
| description | The antioxidant and metal chelating activities in J. curcas protein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. Peptidic fractions with higher radical scavenging activity were analysed by matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant activity was determined by measuring inhibition of the oxidative degradation of β-carotene and by measuring the reactive oxygen species (ROS) in Caco-2 cell cultures. Cu2+ and Fe2+ chelating activities were also determined. The hydrolysates inhibited the degradation of β-carotene and the formation of ROS in Caco-2 cells. The lower molecular weight peptidic fractions from FPLC had stronger antioxidant activity in cell cultures compared with the hydrolysates, which correlated with a higher content in antioxidant and chelating amino acids. These fractions were characterized by a large presence of peptides with different molecular masses. The hydrolysates exhibited both Cu2+ and Fe2+ chelating activity. It was concluded that J. curcas is a good source of antioxidant and metal chelating peptides, which may have a positive impact on the economic value of this crop, as a potential source of food functional components. |
| format | Article |
| id | doaj-art-4bd23c371d7f4cdea8093ab8050a67bc |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-4bd23c371d7f4cdea8093ab8050a67bc2025-02-03T01:02:00ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/190129190129Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and PancreatinSantiago Gallegos Tintoré0Cristina Torres Fuentes1Javier Solorza Feria2Manuel Alaiz3Julio Girón Calle4Alma Leticia Martínez Ayala5Luis Chel Guerrero6Javier Vioque7Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Km 6 Carretera Yautepec-Jojutla, Calle Ceprobi 8, Col. San Isidro, 62731 Yautepec, MOR, MexicoInstituto de la Grasa CSIC, Avenida Padre García Tejero 4, 41012 Sevilla, SpainCentro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Km 6 Carretera Yautepec-Jojutla, Calle Ceprobi 8, Col. San Isidro, 62731 Yautepec, MOR, MexicoInstituto de la Grasa CSIC, Avenida Padre García Tejero 4, 41012 Sevilla, SpainInstituto de la Grasa CSIC, Avenida Padre García Tejero 4, 41012 Sevilla, SpainCentro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Km 6 Carretera Yautepec-Jojutla, Calle Ceprobi 8, Col. San Isidro, 62731 Yautepec, MOR, MexicoFacultad de Ingeniería Química, Universidad Autónoma de Yucatán, Campus de Ingenierías y Ciencias Exactas, Periférico Norte km 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, 97203 Mérida, YUC, MexicoInstituto de la Grasa CSIC, Avenida Padre García Tejero 4, 41012 Sevilla, SpainThe antioxidant and metal chelating activities in J. curcas protein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. Peptidic fractions with higher radical scavenging activity were analysed by matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant activity was determined by measuring inhibition of the oxidative degradation of β-carotene and by measuring the reactive oxygen species (ROS) in Caco-2 cell cultures. Cu2+ and Fe2+ chelating activities were also determined. The hydrolysates inhibited the degradation of β-carotene and the formation of ROS in Caco-2 cells. The lower molecular weight peptidic fractions from FPLC had stronger antioxidant activity in cell cultures compared with the hydrolysates, which correlated with a higher content in antioxidant and chelating amino acids. These fractions were characterized by a large presence of peptides with different molecular masses. The hydrolysates exhibited both Cu2+ and Fe2+ chelating activity. It was concluded that J. curcas is a good source of antioxidant and metal chelating peptides, which may have a positive impact on the economic value of this crop, as a potential source of food functional components.http://dx.doi.org/10.1155/2015/190129 |
| spellingShingle | Santiago Gallegos Tintoré Cristina Torres Fuentes Javier Solorza Feria Manuel Alaiz Julio Girón Calle Alma Leticia Martínez Ayala Luis Chel Guerrero Javier Vioque Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin Journal of Chemistry |
| title | Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin |
| title_full | Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin |
| title_fullStr | Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin |
| title_full_unstemmed | Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin |
| title_short | Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin |
| title_sort | antioxidant and chelating activity of nontoxic jatropha curcas l protein hydrolysates produced by in vitro digestion using pepsin and pancreatin |
| url | http://dx.doi.org/10.1155/2015/190129 |
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