Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat
Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/10/1538 |
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| Summary: | Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance genes from the relatives of wheat. <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey has been widely hybridized with common wheat and has been shown to be a valuable source of genes, conferring resistance and tolerance against both the biotic and abiotic stresses affecting wheat. In this study, a novel wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L addition line, named Th93-1-6, which originated from wheat–<i>Th. intermedium</i> partial amphidiploid line, Th24-19-5, was comprehensively characterized using nondenaturing-fluorescence <i>in situ</i> hybridization (ND-FISH) and Oligo-FISH painting techniques. To detect plants with the transfer of resistance genes from Th93-1-6 to wheat chromosomes, 2384 M<sub>1</sub>-M<sub>3</sub> plants from the cross between Th93-1-6 and the susceptible wheat cultivar MY11 were studied by ND-FISH using multiple probes. A total of 37 types of 2StS.2J<sup>S</sup>L chromosomal aberrations were identified. Subsequently, 12 homozygous lines were developed to construct a cytological bin map. Ten chromosomal bins on the 2StS.2J<sup>S</sup>L chromosome were constructed based on 84 specific molecular markers. Among them, eight alien chromosome aberration lines, which all contained the bin 2StS-3, showed enhanced stripe rust resistance. Consequently, the gene(s) for stripe rust resistance was physically mapped to the 92.88-155.32 Mb region of 2StS in <i>Thinopyrum intermedium</i> reference genome sequences v2.1. Moreover, these newly developed wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L translocation lines are expected to serve as valuable genetic resources in the breeding of rust-resistant wheat cultivars. |
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| ISSN: | 2223-7747 |