An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus)
Abstract Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04523-w |
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| author | Yu Chen Xinghua Lin Dayan Zhou Yulei Zhang Qian Wang Huapu Chen Dongneng Jiang Siping Deng Guangli Li Peipei Wang Changxu Tian |
| author_facet | Yu Chen Xinghua Lin Dayan Zhou Yulei Zhang Qian Wang Huapu Chen Dongneng Jiang Siping Deng Guangli Li Peipei Wang Changxu Tian |
| author_sort | Yu Chen |
| collection | DOAJ |
| description | Abstract Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using MGI, PacBio, and Hi-C sequencing technologies. The final genome assembly spans 982.84 Mb, with contig and scaffold N50 values of 36.16 Mb and 37.66 Mb, respectively, and successfully anchors 99.60% of the sequences to 28 pseudochromosomes. We also predicted 24,849 protein-coding genes, with 97.3% of them functionally annotated. BUSCO analysis indicates a completeness of 97.03% for the assembly and 96.6% for the annotation. This study significantly advances the genomic resources available for C. fuscus and supports future molecular breeding and functional genomics research. |
| format | Article |
| id | doaj-art-4a8d1568049c4b05984cbc14290768db |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-4a8d1568049c4b05984cbc14290768db2025-02-02T12:07:59ZengNature PortfolioScientific Data2052-44632025-02-0112111010.1038/s41597-025-04523-wAn improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus)Yu Chen0Xinghua Lin1Dayan Zhou2Yulei Zhang3Qian Wang4Huapu Chen5Dongneng Jiang6Siping Deng7Guangli Li8Peipei Wang9Changxu Tian10Fisheries College, Guangdong Ocean UniversityFisheries College, Guangdong Ocean UniversityGuangxi Introduction and Breeding Center of AquacultureFisheries College, Guangdong Ocean UniversityCollege of Animal Science and Technology, Yangtze UniversityFisheries College, Guangdong Ocean UniversityFisheries College, Guangdong Ocean UniversityFisheries College, Guangdong Ocean UniversityFisheries College, Guangdong Ocean UniversityGuangxi Introduction and Breeding Center of AquacultureFisheries College, Guangdong Ocean UniversityAbstract Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using MGI, PacBio, and Hi-C sequencing technologies. The final genome assembly spans 982.84 Mb, with contig and scaffold N50 values of 36.16 Mb and 37.66 Mb, respectively, and successfully anchors 99.60% of the sequences to 28 pseudochromosomes. We also predicted 24,849 protein-coding genes, with 97.3% of them functionally annotated. BUSCO analysis indicates a completeness of 97.03% for the assembly and 96.6% for the annotation. This study significantly advances the genomic resources available for C. fuscus and supports future molecular breeding and functional genomics research.https://doi.org/10.1038/s41597-025-04523-w |
| spellingShingle | Yu Chen Xinghua Lin Dayan Zhou Yulei Zhang Qian Wang Huapu Chen Dongneng Jiang Siping Deng Guangli Li Peipei Wang Changxu Tian An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) Scientific Data |
| title | An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) |
| title_full | An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) |
| title_fullStr | An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) |
| title_full_unstemmed | An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) |
| title_short | An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus) |
| title_sort | improved chromosome level genome assembly and annotation of hong kong catfish clarias fuscus |
| url | https://doi.org/10.1038/s41597-025-04523-w |
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