An improved chromosome-level genome assembly and annotation of Hong Kong catfish (Clarias fuscus)

Abstract Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using...

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Main Authors: Yu Chen, Xinghua Lin, Dayan Zhou, Yulei Zhang, Qian Wang, Huapu Chen, Dongneng Jiang, Siping Deng, Guangli Li, Peipei Wang, Changxu Tian
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04523-w
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Summary:Abstract Clarias fuscus, renowned for its resilience and nutritional value, is a significant aquaculture species in China. To facilitate further genetic research and breeding programs in this species, we generated an improved high-quality chromosome-level genome assembly of a female C. fuscus using MGI, PacBio, and Hi-C sequencing technologies. The final genome assembly spans 982.84 Mb, with contig and scaffold N50 values of 36.16 Mb and 37.66 Mb, respectively, and successfully anchors 99.60% of the sequences to 28 pseudochromosomes. We also predicted 24,849 protein-coding genes, with 97.3% of them functionally annotated. BUSCO analysis indicates a completeness of 97.03% for the assembly and 96.6% for the annotation. This study significantly advances the genomic resources available for C. fuscus and supports future molecular breeding and functional genomics research.
ISSN:2052-4463