Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

ABSTRACT We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion membrane protein, as shown with Western...

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Bibliographic Details
Main Authors: Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:mBio
Subjects:
engineered sRNA
protein depletion
operon
essential genes
genetics
Online Access:https://journals.asm.org/doi/10.1128/mbio.02545-24
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