DEL phenotype in RhD-negative North Indian blood donors
BACKGROUND: Rh-DEL type is not detected on routine serology and requires specialized adsorption elution methods which are laborious. Identifying the DEL phenotype in blood donors is important to prevent alloimmunization in transfusion recipients. The present study aimed to determine the frequency of...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-01-01
|
| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ajts.ajts_127_23 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593726130618368 |
|---|---|
| author | Paramjit Kaur Ravneet K. Bedi Tanvi Sood Kshitija Mittal Gagandeep Kaur |
| author_facet | Paramjit Kaur Ravneet K. Bedi Tanvi Sood Kshitija Mittal Gagandeep Kaur |
| author_sort | Paramjit Kaur |
| collection | DOAJ |
| description | BACKGROUND:
Rh-DEL type is not detected on routine serology and requires specialized adsorption elution methods which are laborious. Identifying the DEL phenotype in blood donors is important to prevent alloimmunization in transfusion recipients. The present study aimed to determine the frequency of DEL phenotype in RhD-negative North Indian blood donors and correlate the results with Rh Cc/Ee phenotype.
MATERIALS AND METHODS:
In this prospective descriptive cross-sectional study, a total of 205 blood donors with historic blood group RhD-negative were enrolled. All samples were subjected to blood grouping using a fully automated immunohematology analyzer and samples that typed as RhD negative by two different anti-D antisera were tested for Weak D. Weak D-negative samples were subjected to adsorption and elution for DEL phenotype. All samples were also tested for extended Rh phenotype for C/c and E/e antigens.
RESULTS:
Of the total 11934 donors during the study, 6.2% (n = 743) donors were RhD negative. Of the 205 donors enrolled in the study, two donor samples were serologically weak D positive. None of the remaining 203 donors tested positive for the DEL phenotype. The extended Rh phenotype performed for these donors showed that 6.83% (n = 14) donors were positive for RhC antigen and 1.46% (n = 3) were positive for Rh E antigen. Both weak D-positive donors were also positive for the Rh C antigen.
CONCLUSION:
The prevalence of DEL phenotype is low in the Indian population and studies with larger sample sizes are required to determine the effectiveness of routine C/E typing as a strategy to identify DEL-positive individuals. |
| format | Article |
| id | doaj-art-48c182dfb6554c0db1a1c3cc3fa38910 |
| institution | Kabale University |
| issn | 0973-6247 1998-3565 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj-art-48c182dfb6554c0db1a1c3cc3fa389102025-01-20T09:22:05ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652024-01-01181162010.4103/ajts.ajts_127_23DEL phenotype in RhD-negative North Indian blood donorsParamjit KaurRavneet K. BediTanvi SoodKshitija MittalGagandeep KaurBACKGROUND: Rh-DEL type is not detected on routine serology and requires specialized adsorption elution methods which are laborious. Identifying the DEL phenotype in blood donors is important to prevent alloimmunization in transfusion recipients. The present study aimed to determine the frequency of DEL phenotype in RhD-negative North Indian blood donors and correlate the results with Rh Cc/Ee phenotype. MATERIALS AND METHODS: In this prospective descriptive cross-sectional study, a total of 205 blood donors with historic blood group RhD-negative were enrolled. All samples were subjected to blood grouping using a fully automated immunohematology analyzer and samples that typed as RhD negative by two different anti-D antisera were tested for Weak D. Weak D-negative samples were subjected to adsorption and elution for DEL phenotype. All samples were also tested for extended Rh phenotype for C/c and E/e antigens. RESULTS: Of the total 11934 donors during the study, 6.2% (n = 743) donors were RhD negative. Of the 205 donors enrolled in the study, two donor samples were serologically weak D positive. None of the remaining 203 donors tested positive for the DEL phenotype. The extended Rh phenotype performed for these donors showed that 6.83% (n = 14) donors were positive for RhC antigen and 1.46% (n = 3) were positive for Rh E antigen. Both weak D-positive donors were also positive for the Rh C antigen. CONCLUSION: The prevalence of DEL phenotype is low in the Indian population and studies with larger sample sizes are required to determine the effectiveness of routine C/E typing as a strategy to identify DEL-positive individuals.https://journals.lww.com/10.4103/ajts.ajts_127_23adsorption-elutiondel phenotyperh d variants |
| spellingShingle | Paramjit Kaur Ravneet K. Bedi Tanvi Sood Kshitija Mittal Gagandeep Kaur DEL phenotype in RhD-negative North Indian blood donors Asian Journal of Transfusion Science adsorption-elution del phenotype rh d variants |
| title | DEL phenotype in RhD-negative North Indian blood donors |
| title_full | DEL phenotype in RhD-negative North Indian blood donors |
| title_fullStr | DEL phenotype in RhD-negative North Indian blood donors |
| title_full_unstemmed | DEL phenotype in RhD-negative North Indian blood donors |
| title_short | DEL phenotype in RhD-negative North Indian blood donors |
| title_sort | del phenotype in rhd negative north indian blood donors |
| topic | adsorption-elution del phenotype rh d variants |
| url | https://journals.lww.com/10.4103/ajts.ajts_127_23 |
| work_keys_str_mv | AT paramjitkaur delphenotypeinrhdnegativenorthindianblooddonors AT ravneetkbedi delphenotypeinrhdnegativenorthindianblooddonors AT tanvisood delphenotypeinrhdnegativenorthindianblooddonors AT kshitijamittal delphenotypeinrhdnegativenorthindianblooddonors AT gagandeepkaur delphenotypeinrhdnegativenorthindianblooddonors |