环尾狐猴源弓形虫多位点基因的克隆、测序及基因分型
为确定引起广东环尾狐猴(<i>Lemur catta</i>)疑似弓形虫病的病原,从2只死亡的环尾狐猴体内无菌采集肺脏、肝脏、心、肾和脑等组织制作常规石蜡切片,HE染色。通过病理组织学观察,鉴定为弓形虫(<i>Toxoplasma</i> sp.)后,采用PCR-DNA测序分析方法对环尾狐猴源分离虫株(TgRTL1)的SAG1、SAG2、SAG3、BTUB、GRA6、L358、PK1、c22-8、c29-2和Apico基因位点进行PCR扩增、克隆和测序,采用DNAStar和MEGA5.05软件,将所获序列与网上下载的弓形虫RH株、ME49株和VE...
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Chinese Journal of Wildlife
2017-01-01
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| Series: | 野生动物学报 |
| Subjects: | |
| Online Access: | http://ysdw.nefu.edu.cn/thesisDetails?columnId=67070195&Fpath=home&index=0 |
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