Alterations in Monocyte CD16 in Association with Diabetes Complications
Monocytes express many cell surface markers indicative of their inflammatory and activation status. Whether these markers are affected by diabetes and its complications is not known and was investigated in this study. Blood was obtained from 22 nondiabetic and 43 diabetic subjects with a duration o...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/649083 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567648068567040 |
|---|---|
| author | Danqing Min Belinda Brooks Jencia Wong Robert Salomon Wensheng Bao Brian Harrisberg Stephen M. Twigg Dennis K. Yue Susan V. McLennan |
| author_facet | Danqing Min Belinda Brooks Jencia Wong Robert Salomon Wensheng Bao Brian Harrisberg Stephen M. Twigg Dennis K. Yue Susan V. McLennan |
| author_sort | Danqing Min |
| collection | DOAJ |
| description | Monocytes express many cell surface markers indicative of their inflammatory and activation status. Whether these markers are affected by diabetes and its complications is not known and was investigated in this study. Blood was obtained from 22 nondiabetic and 43 diabetic subjects with a duration of diabetes >10 years, including 25 without and 18 with clinically significant complications. The number of CD45+CD14+ monocytes and the percentage expressing the proinflammatory marker CD16 were determined by flow cytometry. Other markers of monocyte activation and expression of chemokine receptors were also examined. The relationship between monocyte CD16 and clinical data, selected cytokines, and chemokines was also investigated. Diabetes had no effect on total white cell number but increased monocyte number. Diabetes also significantly decreased the number of CD16+ monocytes but only in those with diabetic complications. Other markers of monocyte activation status and chemokine receptors were not affected by diabetes or complications status. Diabetes induced plasma proinflammatory cytokines and they were lower in diabetic subjects with complications compared to those without complications. These results suggest that the circulating monocyte phenotype is altered by diabetic complications status. These changes may be causally related to and could potentially be used to predict susceptibility to diabetic complications. |
| format | Article |
| id | doaj-art-47ffae53d75f4324b2252bebc4e1e771 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-47ffae53d75f4324b2252bebc4e1e7712025-02-03T01:00:54ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/649083649083Alterations in Monocyte CD16 in Association with Diabetes ComplicationsDanqing Min0Belinda Brooks1Jencia Wong2Robert Salomon3Wensheng Bao4Brian Harrisberg5Stephen M. Twigg6Dennis K. Yue7Susan V. McLennan8Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaDiabetes Centre, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaDiscipline of Medicine and Bosch Institute, The University of Sydney, Sydney, NSW 2006, AustraliaDiscipline of Medicine and Bosch Institute, The University of Sydney, Sydney, NSW 2006, AustraliaDiscipline of Medicine and Bosch Institute, The University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Ophthalmology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaDepartment of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaDepartment of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaDepartment of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaMonocytes express many cell surface markers indicative of their inflammatory and activation status. Whether these markers are affected by diabetes and its complications is not known and was investigated in this study. Blood was obtained from 22 nondiabetic and 43 diabetic subjects with a duration of diabetes >10 years, including 25 without and 18 with clinically significant complications. The number of CD45+CD14+ monocytes and the percentage expressing the proinflammatory marker CD16 were determined by flow cytometry. Other markers of monocyte activation and expression of chemokine receptors were also examined. The relationship between monocyte CD16 and clinical data, selected cytokines, and chemokines was also investigated. Diabetes had no effect on total white cell number but increased monocyte number. Diabetes also significantly decreased the number of CD16+ monocytes but only in those with diabetic complications. Other markers of monocyte activation status and chemokine receptors were not affected by diabetes or complications status. Diabetes induced plasma proinflammatory cytokines and they were lower in diabetic subjects with complications compared to those without complications. These results suggest that the circulating monocyte phenotype is altered by diabetic complications status. These changes may be causally related to and could potentially be used to predict susceptibility to diabetic complications.http://dx.doi.org/10.1155/2012/649083 |
| spellingShingle | Danqing Min Belinda Brooks Jencia Wong Robert Salomon Wensheng Bao Brian Harrisberg Stephen M. Twigg Dennis K. Yue Susan V. McLennan Alterations in Monocyte CD16 in Association with Diabetes Complications Mediators of Inflammation |
| title | Alterations in Monocyte CD16 in Association with Diabetes Complications |
| title_full | Alterations in Monocyte CD16 in Association with Diabetes Complications |
| title_fullStr | Alterations in Monocyte CD16 in Association with Diabetes Complications |
| title_full_unstemmed | Alterations in Monocyte CD16 in Association with Diabetes Complications |
| title_short | Alterations in Monocyte CD16 in Association with Diabetes Complications |
| title_sort | alterations in monocyte cd16 in association with diabetes complications |
| url | http://dx.doi.org/10.1155/2012/649083 |
| work_keys_str_mv | AT danqingmin alterationsinmonocytecd16inassociationwithdiabetescomplications AT belindabrooks alterationsinmonocytecd16inassociationwithdiabetescomplications AT jenciawong alterationsinmonocytecd16inassociationwithdiabetescomplications AT robertsalomon alterationsinmonocytecd16inassociationwithdiabetescomplications AT wenshengbao alterationsinmonocytecd16inassociationwithdiabetescomplications AT brianharrisberg alterationsinmonocytecd16inassociationwithdiabetescomplications AT stephenmtwigg alterationsinmonocytecd16inassociationwithdiabetescomplications AT denniskyue alterationsinmonocytecd16inassociationwithdiabetescomplications AT susanvmclennan alterationsinmonocytecd16inassociationwithdiabetescomplications |