Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides
This study investigated the structural characteristics and antioxidant activity of Polygonatum kingianum Coll.et Hemsl polysaccharides (NPKP) processed via nine-steaming-nine-drying methods. Monosaccharide composition analysis revealed that NPKP consists of rhamnose (3.5%), arabinose (4.6%), galacto...
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Academy of National Food and Strategic Reserves Administration
2025-01-01
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| Series: | Liang you shipin ke-ji |
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| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250119 |
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| author | LIU Yu-bin CHEN Chang-an SU Yi-lin LUO Zhen YAN Jin LI Pan DU Bing |
| author_facet | LIU Yu-bin CHEN Chang-an SU Yi-lin LUO Zhen YAN Jin LI Pan DU Bing |
| author_sort | LIU Yu-bin |
| collection | DOAJ |
| description | This study investigated the structural characteristics and antioxidant activity of Polygonatum kingianum Coll.et Hemsl polysaccharides (NPKP) processed via nine-steaming-nine-drying methods. Monosaccharide composition analysis revealed that NPKP consists of rhamnose (3.5%), arabinose (4.6%), galactose (78.2%), glucose (2.1%), xylose (1.0%), mannose (1.3%), and galacturonic acid (9.4%). Molecular weight analysis revealed two main components in NPKP, with molecular weights of 196,857 Da and 13,653 Da. Methylation analysis indicated that NPKP is primarily linked by →4)-Glap-(1→ glycosidic bonds, accounting for 31.8% of the total, with a branching degree of 51.7%. Additionally, iodine-potassium iodide tests confirmed that NPKP is a highly branched polysaccharide. In vitro cellular experiments showed that NPKP significantly reduced reactive oxygen species (ROS) levels in HepG2 cells. It also upregulated antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), alleviating oxidative stress induced by free fatty acids. Through these mechanisms, NPKP exhibited remarkable antioxidant capacity. This study provides a basis data for the application of Polygonatum polysaccharides as natural antioxidants and supports their role in mitigating oxidative stress-related diseases. |
| format | Article |
| id | doaj-art-4682025280a94250a3db8e2f77014d2d |
| institution | Kabale University |
| issn | 1007-7561 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
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| series | Liang you shipin ke-ji |
| spelling | doaj-art-4682025280a94250a3db8e2f77014d2d2025-01-23T14:36:18ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612025-01-0133117418210.16210/j.cnki.1007-7561.2025.01.018Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl PolysaccharidesLIU Yu-bin0CHEN Chang-an1SU Yi-lin2LUO Zhen3YAN Jin4LI Pan5 DU Bing6Infinitus (China) Co., Ltd., Guangzhou, Guangdong 510665, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaInfinitus (China) Co., Ltd., Guangzhou, Guangdong 510665, ChinaInfinitus (China) Co., Ltd., Guangzhou, Guangdong 510665, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaThis study investigated the structural characteristics and antioxidant activity of Polygonatum kingianum Coll.et Hemsl polysaccharides (NPKP) processed via nine-steaming-nine-drying methods. Monosaccharide composition analysis revealed that NPKP consists of rhamnose (3.5%), arabinose (4.6%), galactose (78.2%), glucose (2.1%), xylose (1.0%), mannose (1.3%), and galacturonic acid (9.4%). Molecular weight analysis revealed two main components in NPKP, with molecular weights of 196,857 Da and 13,653 Da. Methylation analysis indicated that NPKP is primarily linked by →4)-Glap-(1→ glycosidic bonds, accounting for 31.8% of the total, with a branching degree of 51.7%. Additionally, iodine-potassium iodide tests confirmed that NPKP is a highly branched polysaccharide. In vitro cellular experiments showed that NPKP significantly reduced reactive oxygen species (ROS) levels in HepG2 cells. It also upregulated antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), alleviating oxidative stress induced by free fatty acids. Through these mechanisms, NPKP exhibited remarkable antioxidant capacity. This study provides a basis data for the application of Polygonatum polysaccharides as natural antioxidants and supports their role in mitigating oxidative stress-related diseases.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250119nine-steaming-nine-processing polygonatum kingianum coll.et hemslpolygonatum kingianum coll. et. hemsl. polysaccharidepolysaccharide structureoxidative stressantioxidant |
| spellingShingle | LIU Yu-bin CHEN Chang-an SU Yi-lin LUO Zhen YAN Jin LI Pan DU Bing Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides Liang you shipin ke-ji nine-steaming-nine-processing polygonatum kingianum coll.et hemsl polygonatum kingianum coll. et. hemsl. polysaccharide polysaccharide structure oxidative stress antioxidant |
| title | Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides |
| title_full | Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides |
| title_fullStr | Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides |
| title_full_unstemmed | Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides |
| title_short | Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides |
| title_sort | structural characteristics and inhibiting oxidative stress of nine steaming nine processing polygonatum kingianum coll et hemsl polysaccharides |
| topic | nine-steaming-nine-processing polygonatum kingianum coll.et hemsl polygonatum kingianum coll. et. hemsl. polysaccharide polysaccharide structure oxidative stress antioxidant |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250119 |
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