Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss.
Silene sendtneri Boiss. (Caryophyllaceae) is the Dinaric endemic plant species with white, decorative and scented flowers. Previous studies on this endemic species were based on morphology and effects on seed germination after the treatment with salicylic acid. However, no molecular genetic studies...
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Faculty of Forestry, University of Banja Luka
2021-12-01
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| Series: | Glasnik Šumarskog Fakulteta Univerziteta u Banjoj Luci |
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| Online Access: | http://filozofskopisanje.com/ojs_test/index.php/gsfbl/article/view/246 |
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| author | Jasna Hanjalić Lejla Ušanović Lejla Lasić Abdurahim Kalajdžić Belma Kalamujić Stroil Adisa Ahmić Naris Pojskić |
| author_facet | Jasna Hanjalić Lejla Ušanović Lejla Lasić Abdurahim Kalajdžić Belma Kalamujić Stroil Adisa Ahmić Naris Pojskić |
| author_sort | Jasna Hanjalić |
| collection | DOAJ |
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Silene sendtneri Boiss. (Caryophyllaceae) is the Dinaric endemic plant species with white, decorative and scented flowers. Previous studies on this endemic species were based on morphology and effects on seed germination after the treatment with salicylic acid. However, no molecular genetic studies have been conducted on this species so far. This paper presents preliminary results of the usefulness of microsatellite loci created for cosmopolitan species in assessing the genetic diversity of endemic plant species. A total of 100 specimens were collected from 18 localities in the mountain regions of Treskavica, Igman, Bjelašnica and Ozren in Bosnia and Herzegovina. No S. sendtneri individuals were found at the mountain Trebević. We tested cross-amplification success and a polymorphism level for the set of microsatellite markers (Sil01, Sil03, Sil16, Sil31, Sil35) designed for the cosmopolitan species Silene nutans. In 100 analyzed individuals of S. sendtneri, Sil31 and Sil35 did not amplify, Sil01 was monomorphic and the remaining two loci showed a high level of allelic diversity. Our findings suggest that caution should therefore be exercised in selecting microsatellite markers designed for cosmopolitan plant species in the analyses of endemic species of the same genus since different genetic factors affect the amplification success and polymorphism of the given loci. Attention should be given to the number of detected and effective alleles and their ratio, the success of locus amplification concerning the complete set of markers used, and the ratio of polymorphs to the total number of observed loci.
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| format | Article |
| id | doaj-art-45e7ab11c63c4680bd5ed9b7b3c462ae |
| institution | Kabale University |
| issn | 1512-956X 2303-694X |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Faculty of Forestry, University of Banja Luka |
| record_format | Article |
| series | Glasnik Šumarskog Fakulteta Univerziteta u Banjoj Luci |
| spelling | doaj-art-45e7ab11c63c4680bd5ed9b7b3c462ae2025-01-27T06:20:22ZengFaculty of Forestry, University of Banja LukaGlasnik Šumarskog Fakulteta Univerziteta u Banjoj Luci1512-956X2303-694X2021-12-0131Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss.Jasna Hanjalić0Lejla Ušanović1Lejla Lasić2Abdurahim Kalajdžić3Belma Kalamujić Stroil4Adisa Ahmić5Naris Pojskić6University of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaUniversity of Tuzla-Department of Biology, Faculty of Natural Sciences, Univerzitetska 4, 75000 Tuzla, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina Silene sendtneri Boiss. (Caryophyllaceae) is the Dinaric endemic plant species with white, decorative and scented flowers. Previous studies on this endemic species were based on morphology and effects on seed germination after the treatment with salicylic acid. However, no molecular genetic studies have been conducted on this species so far. This paper presents preliminary results of the usefulness of microsatellite loci created for cosmopolitan species in assessing the genetic diversity of endemic plant species. A total of 100 specimens were collected from 18 localities in the mountain regions of Treskavica, Igman, Bjelašnica and Ozren in Bosnia and Herzegovina. No S. sendtneri individuals were found at the mountain Trebević. We tested cross-amplification success and a polymorphism level for the set of microsatellite markers (Sil01, Sil03, Sil16, Sil31, Sil35) designed for the cosmopolitan species Silene nutans. In 100 analyzed individuals of S. sendtneri, Sil31 and Sil35 did not amplify, Sil01 was monomorphic and the remaining two loci showed a high level of allelic diversity. Our findings suggest that caution should therefore be exercised in selecting microsatellite markers designed for cosmopolitan plant species in the analyses of endemic species of the same genus since different genetic factors affect the amplification success and polymorphism of the given loci. Attention should be given to the number of detected and effective alleles and their ratio, the success of locus amplification concerning the complete set of markers used, and the ratio of polymorphs to the total number of observed loci. http://filozofskopisanje.com/ojs_test/index.php/gsfbl/article/view/246cosmopolitan speciesdiversityendemic speciesmicrosatelliteSilene sendtneri |
| spellingShingle | Jasna Hanjalić Lejla Ušanović Lejla Lasić Abdurahim Kalajdžić Belma Kalamujić Stroil Adisa Ahmić Naris Pojskić Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. Glasnik Šumarskog Fakulteta Univerziteta u Banjoj Luci cosmopolitan species diversity endemic species microsatellite Silene sendtneri |
| title | Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. |
| title_full | Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. |
| title_fullStr | Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. |
| title_full_unstemmed | Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. |
| title_short | Аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species: a case study of Silene sendtneri Boiss. |
| title_sort | аpplicability of microsatellite loci designed for cosmopolitan species for the investigation of endemic species a case study of silene sendtneri boiss |
| topic | cosmopolitan species diversity endemic species microsatellite Silene sendtneri |
| url | http://filozofskopisanje.com/ojs_test/index.php/gsfbl/article/view/246 |
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