Circular permutation of red fluorescent proteins.

Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) b...

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Bibliographic Details
Main Authors: Bo Shui, Qi Wang, Frank Lee, Laura J Byrnes, Dmitry M Chudakov, Sergey A Lukyanov, Holger Sondermann, Michael I Kotlikoff
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0020505&type=printable
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